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==The AvrPtoB-BAK1 complex reveals two structurally similar kinaseinteracting domains in a single type III effector==
==The AvrPtoB-BAK1 complex reveals two structurally similar kinaseinteracting domains in a single type III effector==
<StructureSection load='3tl8' size='340' side='right' caption='[[3tl8]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='3tl8' size='340' side='right'caption='[[3tl8]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3tl8]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._tomato Pseudomonas syringae pv. tomato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TL8 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3tl8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._tomato Pseudomonas syringae pv. tomato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TL8 FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BAK1, ELG, SERK3, At4g33430, F17M5.190 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), hopAB2, avrPtoB, PSPTO_3087 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=323 Pseudomonas syringae pv. tomato])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tl8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tl8 RCSB], [http://www.ebi.ac.uk/pdbsum/3tl8 PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tl8 OCA], [https://pdbe.org/3tl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tl8 RCSB], [https://www.ebi.ac.uk/pdbsum/3tl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tl8 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BAK1_ARATH BAK1_ARATH] Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Controls the expression of genes associated with innate immunity in the absence of pathogens or elicitors. Involved in brassinosteroid (BR) signal transduction. Phosphorylates BRI1. May be involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-BAK1 heterodimers. Interaction with MSBP1 stimulates the endocytosis of BAK1 and suppresses brassinosteroid signaling. Acts in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) via its interaction with FLS2 and the phosphorylation of BIK1. Involved in programmed cell death (PCD) control. Positively regulates the BR-dependent plant growth pathway and negatively regulates the BR-independent cell-death pathway.<ref>PMID:17600708</ref> <ref>PMID:17583510</ref> <ref>PMID:18694562</ref> <ref>PMID:18667726</ref> <ref>PMID:19124768</ref> <ref>PMID:20018402</ref> <ref>PMID:20404519</ref> <ref>PMID:21350342</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
Line 15: Line 18:
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 3tl8" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Avirulence protein|Avirulence protein]]
*[[Avirulence protein 3D structures|Avirulence protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Pseudomonas syringae pv. tomato]]
[[Category: Pseudomonas syringae pv. tomato]]
[[Category: Chai, J]]
[[Category: Chai J]]
[[Category: Cheng, W]]
[[Category: Cheng W]]
[[Category: Gao, H]]
[[Category: Gao H]]
[[Category: Bacterial pathogenesis]]
[[Category: Pamp-triggered immunity]]
[[Category: Plant immunity]]
[[Category: Pseudomonas syringae]]
[[Category: Solanum lycopersicum]]
[[Category: Transferase-ligase complex]]

Latest revision as of 20:33, 1 November 2023

The AvrPtoB-BAK1 complex reveals two structurally similar kinaseinteracting domains in a single type III effectorThe AvrPtoB-BAK1 complex reveals two structurally similar kinaseinteracting domains in a single type III effector

Structural highlights

3tl8 is a 8 chain structure with sequence from Arabidopsis thaliana and Pseudomonas syringae pv. tomato. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BAK1_ARATH Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Controls the expression of genes associated with innate immunity in the absence of pathogens or elicitors. Involved in brassinosteroid (BR) signal transduction. Phosphorylates BRI1. May be involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-BAK1 heterodimers. Interaction with MSBP1 stimulates the endocytosis of BAK1 and suppresses brassinosteroid signaling. Acts in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) via its interaction with FLS2 and the phosphorylation of BIK1. Involved in programmed cell death (PCD) control. Positively regulates the BR-dependent plant growth pathway and negatively regulates the BR-independent cell-death pathway.[1] [2] [3] [4] [5] [6] [7] [8]

Publication Abstract from PubMed

To infect plants, Pseudomonas syringae pv. tomato delivers approximately 30 type III effector proteins into host cells, many of which interfere with PAMP-triggered immunity (PTI). One effector, AvrPtoB, suppresses PTI using a central domain to bind host BAK1, a kinase that acts with several pattern recognition receptors to activate defense signaling. A second AvrPtoB domain binds and suppresses the PTI-associated kinase Bti9 but is conversely recognized by the protein kinase Pto to activate effector-triggered immunity. We report the crystal structure of the AvrPtoB-BAK1 complex, which revealed structural similarity between these two AvrPtoB domains, suggesting that they arose by intragenic duplication. The BAK1 kinase domain is structurally similar to Pto, and a conserved region within both BAK1 and Pto interacts with AvrPtoB. BAK1 kinase activity is inhibited by AvrPtoB, and mutations at the interaction interface disrupt AvrPtoB virulence activity. These results shed light on a structural mechanism underlying host-pathogen coevolution.

Structural Analysis of Pseudomonas syringae AvrPtoB Bound to Host BAK1 Reveals Two Similar Kinase-Interacting Domains in a Type III Effector.,Cheng W, Munkvold KR, Gao H, Mathieu J, Schwizer S, Wang S, Yan YB, Wang J, Martin GB, Chai J Cell Host Microbe. 2011 Dec 15;10(6):616-26. Epub 2011 Dec 8. PMID:22169508[9]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ He K, Gou X, Yuan T, Lin H, Asami T, Yoshida S, Russell SD, Li J. BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell-death pathways. Curr Biol. 2007 Jul 3;17(13):1109-15. PMID:17600708 doi:10.1016/j.cub.2007.05.036
  2. ↑ Kemmerling B, Schwedt A, Rodriguez P, Mazzotta S, Frank M, Qamar SA, Mengiste T, Betsuyaku S, Parker JE, Mussig C, Thomma BP, Albrecht C, de Vries SC, Hirt H, Nurnberger T. The BRI1-associated kinase 1, BAK1, has a brassinolide-independent role in plant cell-death control. Curr Biol. 2007 Jul 3;17(13):1116-22. Epub 2007 Jun 21. PMID:17583510 doi:S0960-9822(07)01470-4
  3. ↑ Wang X, Kota U, He K, Blackburn K, Li J, Goshe MB, Huber SC, Clouse SD. Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in brassinosteroid signaling. Dev Cell. 2008 Aug;15(2):220-35. doi: 10.1016/j.devcel.2008.06.011. PMID:18694562 doi:10.1016/j.devcel.2008.06.011
  4. ↑ Albrecht C, Russinova E, Kemmerling B, Kwaaitaal M, de Vries SC. Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE proteins serve brassinosteroid-dependent and -independent signaling pathways. Plant Physiol. 2008 Sep;148(1):611-9. doi: 10.1104/pp.108.123216. Epub 2008 Jul, 30. PMID:18667726 doi:10.1104/pp.108.123216
  5. ↑ Oh MH, Wang X, Kota U, Goshe MB, Clouse SD, Huber SC. Tyrosine phosphorylation of the BRI1 receptor kinase emerges as a component of brassinosteroid signaling in Arabidopsis. Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):658-63. Epub 2009 Jan 5. PMID:19124768 doi:0810249106
  6. ↑ Postel S, Kufner I, Beuter C, Mazzotta S, Schwedt A, Borlotti A, Halter T, Kemmerling B, Nurnberger T. The multifunctional leucine-rich repeat receptor kinase BAK1 is implicated in Arabidopsis development and immunity. Eur J Cell Biol. 2010 Feb-Mar;89(2-3):169-74. doi: 10.1016/j.ejcb.2009.11.001., Epub 2009 Dec 16. PMID:20018402 doi:10.1016/j.ejcb.2009.11.001
  7. ↑ Lu D, Wu S, He P, Shan L. Phosphorylation of receptor-like cytoplasmic kinases by bacterial flagellin. Plant Signal Behav. 2010 May 9;5(5). PMID:20404519
  8. ↑ Oh MH, Wu X, Clouse SD, Huber SC. Functional importance of BAK1 tyrosine phosphorylation in vivo. Plant Signal Behav. 2011 Mar;6(3):400-5. Epub 2011 Mar 1. PMID:21350342
  9. ↑ Cheng W, Munkvold KR, Gao H, Mathieu J, Schwizer S, Wang S, Yan YB, Wang J, Martin GB, Chai J. Structural Analysis of Pseudomonas syringae AvrPtoB Bound to Host BAK1 Reveals Two Similar Kinase-Interacting Domains in a Type III Effector. Cell Host Microbe. 2011 Dec 15;10(6):616-26. Epub 2011 Dec 8. PMID:22169508 doi:10.1016/j.chom.2011.10.013

3tl8, resolution 2.50Å

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