3s62: Difference between revisions
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==Structure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 protein== | ==Structure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 protein== | ||
<StructureSection load='3s62' size='340' side='right' caption='[[3s62]], [[Resolution|resolution]] 4.01Å' scene=''> | <StructureSection load='3s62' size='340' side='right'caption='[[3s62]], [[Resolution|resolution]] 4.01Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3s62]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3s62]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S62 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.01Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s62 OCA], [https://pdbe.org/3s62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s62 RCSB], [https://www.ebi.ac.uk/pdbsum/3s62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s62 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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==See Also== | ==See Also== | ||
*[[3D structures of antibody|3D structures of antibody]] | *[[Antibody 3D structures|Antibody 3D structures]] | ||
*[[Sandbox 20009|Sandbox 20009]] | |||
*[[3D structures of non-human antibody|3D structures of non-human antibody]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Saxena | [[Category: Saxena AK]] | ||
Latest revision as of 20:23, 1 November 2023
Structure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 proteinStructure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 protein
Structural highlights
Publication Abstract from PubMedUnderstanding the structural basis of recognition between antigen and antibody requires the structural comparison of free and complexed components. Previously, we have reported the crystal structure of the complex between Fab fragment of murine monoclonal antibody 2A8 (Fab2A8) and Plasmodium vivax P25 protein (Pvs25) at 3.2 A resolution. We report here the crystallization and X-ray structure of native Fab2A8 at 4.0 A resolution. The 2A8 antibody generated against Pvs25 prevents the formation of P. vivax oocysts in the mosquito, when assayed in membrane feeding experiment. Comparison of native Fab2A8 structure with antigen bound Fab2A8 structure indicates the significant conformational changes in CDR-H1 and CDR-H3 regions of V(H) domain and CDR-L3 region of V(L) domain of Fab2A8. Upon complex formation, the relative orientation between V(L) and V(H) domains of Fab2A8 is conserved, while significant differences are observed in elbow angles of heavy and light chains. The combing site residues of complexed Fab2A8 exhibited the reduced temperature factor compared to native Fab2A8, suggesting a loss of conformational entropy upon antigen binding. Structure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 protein.,Saxena AK Int J Biol Macromol. 2012 Jan 1;50(1):153-6. Epub 2011 Oct 20. PMID:22037467[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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