3s62: Difference between revisions

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-[[Image:3s62.jpg|left|200px]]
-<!--
+==Structure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 protein==
-The line below this paragraph, containing "STRUCTURE_3s62", creates the "Structure Box" on the page.
+<StructureSection load='3s62' size='340' side='right'caption='[[3s62]], [[Resolution|resolution]] 4.01&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3s62]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S62 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.01&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s62 OCA], [https://pdbe.org/3s62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s62 RCSB], [https://www.ebi.ac.uk/pdbsum/3s62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s62 ProSAT]</span></td></tr>
-{{STRUCTURE_3s62|  PDB=3s62  |  SCENE=  }}
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Understanding the structural basis of recognition between antigen and antibody requires the structural comparison of free and complexed components. Previously, we have reported the crystal structure of the complex between Fab fragment of murine monoclonal antibody 2A8 (Fab2A8) and Plasmodium vivax P25 protein (Pvs25) at 3.2 A resolution. We report here the crystallization and X-ray structure of native Fab2A8 at 4.0 A resolution. The 2A8 antibody generated against Pvs25 prevents the formation of P. vivax oocysts in the mosquito, when assayed in membrane feeding experiment. Comparison of native Fab2A8 structure with antigen bound Fab2A8 structure indicates the significant conformational changes in CDR-H1 and CDR-H3 regions of V(H) domain and CDR-L3 region of V(L) domain of Fab2A8. Upon complex formation, the relative orientation between V(L) and V(H) domains of Fab2A8 is conserved, while significant differences are observed in elbow angles of heavy and light chains. The combing site residues of complexed Fab2A8 exhibited the reduced temperature factor compared to native Fab2A8, suggesting a loss of conformational entropy upon antigen binding.
-===Structure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 protein===
+Structure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 protein.,Saxena AK Int J Biol Macromol. 2012 Jan 1;50(1):153-6. Epub 2011 Oct 20. PMID:22037467<ref>PMID:22037467</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3s62" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_22037467}}, adds the Publication Abstract to the page
+*[[Antibody 3D structures|Antibody 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 22037467 is the PubMed ID number.
+*[[Sandbox 20009|Sandbox 20009]]
--->
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
-{{ABSTRACT_PUBMED_22037467}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[3s62]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S62 OCA].
+</StructureSection>
+[[Category: Large Structures]]
-==Reference==
-<ref group="xtra">PMID:022037467</ref><references group="xtra"/>
 [[Category: Mus musculus]]
-[[Category: Saxena, A K.]]
+[[Category: Saxena AK]]
-[[Category: Immune system]]
-[[Category: Typical antibody fold]]