3rdz: Difference between revisions

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-[[Image:3rdz.jpg|left|200px]]
-<!--
+==Crystal Structure of rBTI-trypsin complex at 2.26 angstrom resolution==
-The line below this paragraph, containing "STRUCTURE_3rdz", creates the "Structure Box" on the page.
+<StructureSection load='3rdz' size='340' side='right'caption='[[3rdz]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3rdz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Fagopyrum_esculentum Fagopyrum esculentum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RDZ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.262&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-{{STRUCTURE_3rdz|  PDB=3rdz  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rdz OCA], [https://pdbe.org/3rdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rdz RCSB], [https://www.ebi.ac.uk/pdbsum/3rdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rdz ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+BWI-1 (buckwheat trypsin inhibitor), a member of the potato inhibitor I family, suppresses the growth of T-acute lymphoblastic leukemia cells and induces apoptosis in human solid tumor cell lines. Here, we report the crystal structure of rBTI (recombinant buckwheat trypsin inhibitor), a recombinant protein of BWI-1, at 1.84 A resolution and the structure of rBTI in complex with bovine trypsin at 2.26 A resolution. A conformational change of Trp53 at the P(8)' position in rBTI was observed upon its binding to trypsin, which is not seen in other members of the potato inhibitor I family reported previously. The role of the P(8)' residue in the potato inhibitor I family was examined by measuring the association and dissociation rates of four rBTI mutants with different substitutions at the P(2) and P(8)' positions when binding to trypsin. One of the mutants, P44T, was found to be a much stronger inhibitor than wild-type rBTI, with a picomolar (pM) dissociation constant. Our results could provide valuable insights for designing a new rBTI-based antitumor drug in the future.
-===Crystal Structure of rBTI-trypsin complex at 2.26 angstrom resolution===
+Conformational Changes of rBTI from Buckwheat upon Binding to Trypsin: Implications for the Role of the P(8)' Residue in the Potato Inhibitor I Family.,Wang L, Zhao F, Li M, Zhang H, Gao Y, Cao P, Pan X, Wang Z, Chang W PLoS One. 2011;6(6):e20950. Epub 2011 Jun 15. PMID:21698291<ref>PMID:21698291</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3rdz" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21698291}}, adds the Publication Abstract to the page
+*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21698291 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21698291}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[3rdz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Fagopyrum_esculentum Fagopyrum esculentum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RDZ OCA].
-==Reference==
-<ref group="xtra">PMID:021698291</ref><references group="xtra"/>
 [[Category: Bos taurus]]
 [[Category: Fagopyrum esculentum]]
-[[Category: Trypsin]]
+[[Category: Large Structures]]
-[[Category: Chang, W R.]]
+[[Category: Chang WR]]
-[[Category: Li, M.]]
+[[Category: Li M]]
-[[Category: Wang, L F.]]
+[[Category: Wang LF]]
-[[Category: Hydrolase-hydrolase inhibitor complex]]
-[[Category: Potato inhibitor i]]
-[[Category: Serine protease inhibitor]]
-[[Category: Trypsin]]
-[[Category: Trypsin inhibitor]]