3q1x: Difference between revisions

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-[[Image:3q1x.png|left|200px]]
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+==Crystal structure of Entamoeba histolytica serine acetyltransferase 1 in complex with L-serine==
-The line below this paragraph, containing "STRUCTURE_3q1x", creates the "Structure Box" on the page.
+<StructureSection load='3q1x' size='340' side='right'caption='[[3q1x]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3q1x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q1X FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_3q1x|  PDB=3q1x  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q1x OCA], [https://pdbe.org/3q1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q1x RCSB], [https://www.ebi.ac.uk/pdbsum/3q1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q1x ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9U8X2_ENTHI Q9U8X2_ENTHI]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cysteine (cys) plays a major role in growth and survival of the human parasite Entamoeba histolytica. We report here the crystal structure of serine acetyltransferase (SAT) isoform1, a cysteine biosynthetic pathway enzyme, from E. histolytica (EhSAT1) at 1.77 [Aring], in complex with its substrate serine (ser) at 1.59 [Aring] and inhibitor cys at 1.78 [Aring] resolution. EhSAT1 exists as a trimer both in solution as well as in crystal structure, unlike hexamers formed by other known SATs. The difference in oligomeric state is due to the N- terminal region of the EhSAT1, which has very low sequence similarity to known structures, also differs in orientation and charge distribution. The ser and cys bind to the same site, confirming that cys is a competitive inhibitor of ser. The disordered C-terminal region and the loop near the active site are responsible for solvent accessible acetyl Co-A binding site and thus lose inhibition to acetyl Co-A by the feedback inhibitor cys. Docking and fluorescence studies show that EhSAT1 C-terminal mimicking peptides can bind to o-acetyl serine sulfhydrylase (EhOASS), while native C-terminal peptide does not show any binding. To test further, C-terminal end of EhSAT1 was mutated and found that it inhibits EhOASS, confirming modified EhSAT1 can bind to EhOASS. The apparent inability of EhSAT1 to form a hexamer and differences in C-terminal region are likely to be the major reasons for the lack of formation of the large cysteine synthase complex and loss of a complex regulatory mechanism in E. histolytica.
-===Crystal structure of Entamoeba histolytica serine acetyltransferase 1 in complex with L-serine===
+Structural and biochemical studies of serine acetyltransferase reveal why the parasite Entamoeba histolytica cannot form cysteine synthase complex.,Kumar S, Raj I, Nagpal I, Subbarao N, Gourinath S J Biol Chem. 2011 Feb 5. PMID:21297164<ref>PMID:21297164</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3q1x" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21297164}}, adds the Publication Abstract to the page
+*[[Serine acetyltransferase|Serine acetyltransferase]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21297164 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21297164}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[3q1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q1X OCA].
-==Reference==
-<ref group="xtra">PMID:21297164</ref><references group="xtra"/>
 [[Category: Entamoeba histolytica]]
-[[Category: Serine O-acetyltransferase]]
+[[Category: Large Structures]]
-[[Category: Gourinath, S.]]
+[[Category: Gourinath S]]
-[[Category: Kumar, S.]]
+[[Category: Kumar S]]