3puj: Difference between revisions

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-{{STRUCTURE_3puj|  PDB=3puj  |  SCENE=  }}
-===Crystal structure of the MUNC18-1 and SYNTAXIN4 N-Peptide complex===
-{{ABSTRACT_PUBMED_21193638}}
-==Function==
+==Crystal structure of the MUNC18-1 and SYNTAXIN4 N-Peptide complex==
-[[http://www.uniprot.org/uniprot/STXB1_RAT STXB1_RAT]] May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. [[http://www.uniprot.org/uniprot/STX4_MOUSE STX4_MOUSE]] Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.<ref>PMID:9045631</ref> <ref>PMID:10394363</ref> <ref>PMID:18827011</ref>
+<StructureSection load='3puj' size='340' side='right'caption='[[3puj]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3puj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PUJ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.313&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3puj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3puj OCA], [https://pdbe.org/3puj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3puj RCSB], [https://www.ebi.ac.uk/pdbsum/3puj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3puj ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/STXB1_RAT STXB1_RAT] May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Munc18-1 and Syntaxin1 are essential proteins for SNARE-mediated neurotransmission. Munc18-1 participates in synaptic vesicle fusion via dual roles: as a docking/chaperone protein by binding closed Syntaxin1, and as a fusion protein that binds SNARE complexes in a Syntaxin1 N-peptide dependent manner. The two roles are associated with a closed-open Syntaxin1 conformational transition. Here, we show that Syntaxin N-peptide binding to Munc18-1 is not highly selective, suggesting that other parts of the SNARE complex are involved in binding to Munc18-1. We also find that Syntaxin1, with an N peptide and a physically anchored C terminus, binds to Munc18-1 and that this complex can participate in SNARE complex formation. We report a Munc18-1-N-peptide crystal structure that, together with other data, reveals how Munc18-1 might transit from a conformation that binds closed Syntaxin1 to one that may be compatible with binding open Syntaxin1 and SNARE complexes. Our results suggest the possibility that structural transitions occur in both Munc18-1 and Syntaxin1 during their binary interaction. We hypothesize that Munc18-1 domain 3a undergoes a conformational change that may allow coiled-coil interactions with SNARE complexes.
-==About this Structure==
+Possible roles for Munc18-1 domain 3a and Syntaxin1 N-peptide and C-terminal anchor in SNARE complex formation.,Hu SH, Christie MP, Saez NJ, Latham CF, Jarrott R, Lua LH, Collins BM, Martin JL Proc Natl Acad Sci U S A. 2010 Dec 30. PMID:21193638<ref>PMID:21193638</ref>
-[[3puj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PUJ OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:021193638</ref><references group="xtra"/><references/>
+</div>
-[[Category: Buffalo rat]]
+<div class="pdbe-citations 3puj" style="background-color:#fffaf0;"></div>
-[[Category: Christie, M P.]]
-[[Category: Collins, B M.]]
+==See Also==
-[[Category: Hu, S H.]]
+*[[Syntaxin-binding protein|Syntaxin-binding protein]]
-[[Category: Jarrott, R.]]
+== References ==
-[[Category: Latham, C F.]]
+<references/>
-[[Category: Lua, L H.L.]]
+__TOC__
-[[Category: Martin, J L.]]
+</StructureSection>
-[[Category: Saez, N J.]]
+[[Category: Large Structures]]
-[[Category: Endocytosis-exocytosis complex]]
+[[Category: Mus musculus]]
-[[Category: Membrane trafficking]]
+[[Category: Rattus norvegicus]]
-[[Category: Sm protein]]
+[[Category: Christie MP]]
-[[Category: Snare protein]]
+[[Category: Collins BM]]
-[[Category: Syntaxin]]
+[[Category: Hu S-H]]
-[[Category: Syntaxin binding protein]]
+[[Category: Jarrott R]]
+[[Category: Latham CF]]
+[[Category: Lua LHL]]
+[[Category: Martin JL]]
+[[Category: Saez NJ]]