3ppn: Difference between revisions

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-[[Image:3ppn.jpg|left|200px]]
-<!--
+==Structures of the substrate-binding protein provide insights into the multiple compatible solutes binding specificities of Bacillus subtilis ABC transporter OpuC==
-The line below this paragraph, containing "STRUCTURE_3ppn", creates the "Structure Box" on the page.
+<StructureSection load='3ppn' size='340' side='right'caption='[[3ppn]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3ppn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PPN FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ppn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ppn OCA], [https://pdbe.org/3ppn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ppn RCSB], [https://www.ebi.ac.uk/pdbsum/3ppn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ppn ProSAT]</span></td></tr>
-{{STRUCTURE_3ppn|  PDB=3ppn  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/OPUCC_BACSU OPUCC_BACSU] Member of a high affinity multicomponent binding-protein-dependent transport system for glycine betaine, carnitine, and choline.<ref>PMID:10216873</ref> <ref>PMID:8752321</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The compatible solute ATP-binding cassette (ABC) transporters are indispensable for acquiring a variety of compatible solutes under osmotic stress in Bacillus subtilis. The substrate-binding protein OpuCC of the ABC transporter OpuC can recognize a broad spectrum of compatible solutes, compared to its 70% sequence-identical paralog OpuBC that can solely bind choline. To explore the structural basis of this difference of substrate specificity, we determined crystal structures of OpuCC in the apo-form and in complex with carnitine, glycine betaine, choline and ectoine, respectively. OpuCC is composed of two alpha/beta/alpha globular sandwich domains linked by two hinge regions, with substrate-binding pocket located at the inter-domain cleft. Upon substrate-binding, the two domains shift towards each other to trap the substrate. Comparative structural analysis revealed a plastic pocket that fits various compatible solutes, which attributes the multiple-substrate binding property to OpuCC. This plasticity is a gain-of-function via a single-residue mutation of Thr94 in OpuCC versus Asp96 in OpuBC.
-===Structures of the substrate-binding protein provide insights into the multiple compatible solutes binding specificities of Bacillus subtilis ABC transporter OpuC===
+Structures of the substrate-binding protein provide insights into the multiple compatible solutes binding specificities of Bacillus subtilis ABC transporter OpuC.,Du Y, Shi WW, He YX, Yang YH, Zhou CZ, Chen Y Biochem J. 2011 Mar 3. PMID:21366542<ref>PMID:21366542</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3ppn" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21366542}}, adds the Publication Abstract to the page
+*[[ABC transporter 3D structures|ABC transporter 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21366542 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21366542}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[3ppn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPN OCA].
-==Reference==
-<ref group="xtra">PMID:021366542</ref><references group="xtra"/>
 [[Category: Bacillus subtilis]]
-[[Category: Chen, Y.]]
+[[Category: Large Structures]]
-[[Category: Du, Y.]]
+[[Category: Chen Y]]
-[[Category: He, Y X.]]
+[[Category: Du Y]]
-[[Category: Shi, W W.]]
+[[Category: He YX]]
-[[Category: Yang, Y H.]]
+[[Category: Shi WW]]
-[[Category: Zhou, C Z.]]
+[[Category: Yang YH]]
+[[Category: Zhou CZ]]