3p16: Difference between revisions

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-[[Image:3p16.jpg|left|200px]]
-<!--
+==Crystal structure of DNA polymerase III sliding clamp==
-The line below this paragraph, containing "STRUCTURE_3p16", creates the "Structure Box" on the page.
+<StructureSection load='3p16' size='340' side='right'caption='[[3p16]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3p16]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P16 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P16 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.89&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p16 OCA], [https://pdbe.org/3p16 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p16 RCSB], [https://www.ebi.ac.uk/pdbsum/3p16 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p16 ProSAT]</span></td></tr>
-{{STRUCTURE_3p16|  PDB=3p16  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DPO3B_MYCTU DPO3B_MYCTU] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The sliding clamp is a key component of DNA polymerase III (Pol III) required for genome replication. It is known to function with diverse DNA repair proteins and cell cycle-control proteins, making it a potential drug target. To extend our understanding of the structure/function relationship of the sliding clamp, we solved the crystal structure of the sliding clamp from Mycobacterium tuberculosis (M. tuberculosis), a human pathogen that causes most cases of tuberculosis (TB). The sliding clamp from M. tuberculosis forms a ring-shaped head-to-tail dimer with three domains per subunit. Each domain contains two alpha helices in the inner ring that lie against two beta sheets in the outer ring. Previous studies have indicated that many Escherichia coli clamp-binding proteins have a conserved LF sequence, which is critical for binding to the hydrophobic region of the sliding clamp. Here, we analyzed the binding affinities of the M. tuberculosis sliding clamp and peptides derived from the alpha and delta subunits of Pol III, which indicated that the LF motif also plays an important role in the binding of the alpha and delta subunits to the sliding clamp of M. tuberculosis.
-===Crystal structure of DNA polymerase III sliding clamp===
+Crystal structure of DNA polymerase III beta sliding clamp from Mycobacterium tuberculosis.,Gui WJ, Lin SQ, Chen YY, Zhang XE, Bi LJ, Jiang T Biochem Biophys Res Commun. 2011 Feb 11;405(2):272-7. Epub 2011 Jan 8. PMID:21219854<ref>PMID:21219854</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3p16" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21219854}}, adds the Publication Abstract to the page
+*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21219854 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21219854}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[3p16]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P16 OCA].
+[[Category: Mycobacterium tuberculosis H37Rv]]
+[[Category: Bi LJ]]
-==Reference==
+[[Category: Chen YY]]
-<ref group="xtra">PMID:021219854</ref><references group="xtra"/>
+[[Category: Gui WJ]]
-[[Category: DNA-directed DNA polymerase]]
+[[Category: Jiang T]]
-[[Category: Mycobacterium tuberculosis]]
+[[Category: Lin SQ]]
-[[Category: Bi, L J.]]
+[[Category: Zhang XE]]
-[[Category: Chen, Y Y.]]
-[[Category: Gui, W J.]]
-[[Category: Jiang, T.]]
-[[Category: Lin, S Q.]]
-[[Category: Zhang, X E.]]
-[[Category: Dna polymerase iii sliding clamp]]
-[[Category: Transferase]]