3ooh: Difference between revisions

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-[[Image:3ooh.jpg|left|200px]]
-<!--
+==Crystal structure of E. Coli purine nucleoside phosphorylase with PO4==
-The line below this paragraph, containing "STRUCTURE_3ooh", creates the "Structure Box" on the page.
+<StructureSection load='3ooh' size='340' side='right'caption='[[3ooh]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3ooh]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OOH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OOH FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-{{STRUCTURE_3ooh|  PDB=3ooh  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ooh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ooh OCA], [https://pdbe.org/3ooh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ooh RCSB], [https://www.ebi.ac.uk/pdbsum/3ooh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ooh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DEOD_ECOLI DEOD_ECOLI] Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules.[HAMAP-Rule:MF_01627]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The catalytic mechanism of Escherichia coli purine nucleoside phosphorylase (PNP) is revised using site-directed mutagenesis, kinetic studies and structure determinations. The experimental evidence on the role of the particular catalytic amino acid during catalysis has not been available. Therefore, the active site mutants Arg24Ala, Asp204Ala, Asp204Asn, Arg217Ala and Asp204Ala/Arg217Ala were prepared and their kinetics and thermodynamic studies were carried out. The activity tests with natural substrates and 7-methylguanosine confirmed the earlier hypothesis, that catalysis involves protonation of the purine base at position N7 by Asp204, which is triggered by Arg217. The crystal structures of the wild type in complexes with phosphate and sulphate, respectively, and of the Arg24Ala mutant in complex with phosphate/sulphate were determined. The structural data show that previously observed conformational change is a result of the phosphate binding and its interaction with Arg24. As E. coli PNP is a promising candidate for the tumour-directed gene therapy, our results may also help to design efficient mutants useful in gene therapy.
-===Crystal structure of E. Coli purine nucleoside phosphorylase with PO4===
+Validation of the catalytic mechanism of Escherichiacoli purine nucleoside phosphorylase by structural and kinetic studies.,Mikleusevic G, Stefanic Z, Narczyk M, Wielgus-Kutrowska B, Bzowska A, Luic M Biochimie. 2011 Jun 13. PMID:21672603<ref>PMID:21672603</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3ooh" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21672603}}, adds the Publication Abstract to the page
+*[[Purine nucleoside phosphorylase 3D structures|Purine nucleoside phosphorylase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21672603 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21672603}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Escherichia coli K-12]]
-[[3ooh]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OOH OCA].
+[[Category: Large Structures]]
+[[Category: Bzowska A]]
-==Reference==
+[[Category: Luic M]]
-<ref group="xtra">PMID:021672603</ref><references group="xtra"/>
+[[Category: Mikleusevic G]]
-[[Category: Escherichia coli]]
+[[Category: Narzyk M]]
-[[Category: Purine-nucleoside phosphorylase]]
+[[Category: Stefanic Z]]
-[[Category: Bzowska, A.]]
+[[Category: Wielgus-Kutrowska B]]
-[[Category: Luic, M.]]
-[[Category: Mikleusevic, G.]]
-[[Category: Narzyk, M.]]
-[[Category: Stefanic, Z.]]
-[[Category: Wielgus-Kutrowska, B.]]
-[[Category: Phosphorylase]]
-[[Category: Purine nucleoside phosphorylase]]
-[[Category: Transferase]]