3nda: Difference between revisions
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==Crystal structure of serpin from tick Ixodes ricinus== | ==Crystal structure of serpin from tick Ixodes ricinus== | ||
<StructureSection load='3nda' size='340' side='right' caption='[[3nda]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='3nda' size='340' side='right'caption='[[3nda]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3nda]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3nda]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ixodes_ricinus Ixodes ricinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NDA FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nda OCA], [https://pdbe.org/3nda PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nda RCSB], [https://www.ebi.ac.uk/pdbsum/3nda PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nda ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q06B74_IXORI Q06B74_IXORI] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 18: | Line 21: | ||
==See Also== | ==See Also== | ||
*[[Serpin|Serpin]] | *[[Serpin 3D structures|Serpin 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Ixodes ricinus]] | ||
[[Category: Chmelar | [[Category: Large Structures]] | ||
[[Category: Kovarova | [[Category: Chmelar J]] | ||
[[Category: Mares | [[Category: Kovarova Z]] | ||
[[Category: Rezacova | [[Category: Mares M]] | ||
[[Category: Rezacova P]] | |||
Latest revision as of 19:45, 1 November 2023
Crystal structure of serpin from tick Ixodes ricinusCrystal structure of serpin from tick Ixodes ricinus
Structural highlights
FunctionPublication Abstract from PubMedPlatelet aggregation and acute inflammation are key processes in vertebrate defense to a skin injury. Recent studies uncovered the mediation of two serine proteases, cathepsin G and chymase, in both mechanisms. Working with a mouse model of acute inflammation, we revealed that an exogenous salivary protein of Ixodes ricinus, the vector of Lyme disease pathogens in Europe, extensively inhibits edema formation and influx of neutrophils in the inflamed tissue. We named this tick salivary gland secreted effector as Ixodes ricinus serpin-2 (IRS-2) and we show that it primarily inhibits cathepsin G and chymase, while in higher molar excess it affects thrombin activity as well. The inhibitory specificity was explained using the crystal structure, determined at a resolution of 1.8 A. Moreover, we disclosed the ability of IRS-2 to inhibit cathepsin G-induced and thrombin-induced platelet aggregation. For the first time, an ectoparasite protein is shown to exhibit such pharmacological effects and target specificity. The stringent specificity and the biological activities of IRS-2 combined with the knowledge of its structure can be the basis for the development of future pharmaceutical applications. A tick salivary protein targets cathepsin G and chymase and inhibits host inflammation and platelet aggregation.,Chmelar J, Oliveira CJ, Rezacova P, Francischetti IM, Kovarova Z, Pejler G, Kopacek P, Ribeiro JM, Mares M, Kopecky J, Kotsyfakis M Blood. 2010 Oct 12. PMID:20940421[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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