3lt2: Difference between revisions

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-{{Seed}}
-[[Image:3lt2.jpg|left|200px]]
-<!--
+==Enoyl-ACP Reductase from Plasmodium falciparum (PfENR) in complex with triclosan variant T3==
-The line below this paragraph, containing "STRUCTURE_3lt2", creates the "Structure Box" on the page.
+<StructureSection load='3lt2' size='340' side='right'caption='[[3lt2]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3lt2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LT2 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FT3:2-(2,4-DICHLOROPHENOXY)-5-(HYDROXYMETHYL)PHENOL'>FT3</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-{{STRUCTURE_3lt2|  PDB=3lt2  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lt2 OCA], [https://pdbe.org/3lt2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lt2 RCSB], [https://www.ebi.ac.uk/pdbsum/3lt2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lt2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9BJJ9_PLAFA Q9BJJ9_PLAFA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lt/3lt2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lt2 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Triclosan, a well-known inhibitor of Enoyl Acyl Carrier Protein Reductase (ENR) from several pathogenic organisms, is a promising lead compound to design effective drugs. We have solved the X-ray crystal structures of Plasmodium falciparum ENR in complex with triclosan variants having different substituted and unsubstituted groups at different key functional locations. The structures revealed that 4 and 2' substituted compounds have more interactions with the protein, cofactor, and solvents when compared with triclosan. New water molecules were found to interact with some of these inhibitors. Substitution at the 2' position of triclosan caused the relocation of a conserved water molecule, leading to an additional hydrogen bond with the inhibitor. This observation can help in conserved water-based inhibitor design. 2' and 4' unsubstituted compounds showed a movement away from the hydrophobic pocket to compensate for the interactions made by the halogen groups of triclosan. This compound also makes additional interactions with the protein and cofactor which compensate for the lost interactions due to the unsubstitution at 2' and 4'. In cell culture, this inhibitor shows less potency, which indicates that the chlorines at 2' and 4' positions increase the ability of the inhibitor to cross multilayered membranes. This knowledge helps us to modify the different functional groups of triclosan to get more potent inhibitors.
-===Enoyl-ACP Reductase from Plasmodium falciparum (PfENR) in complex with triclosan variant T3===
+X-ray crystallographic analysis of the complexes of enoyl acyl carrier protein reductase of Plasmodium falciparum with triclosan variants to elucidate the importance of different functional groups in enzyme inhibition.,Maity K, Bhargav SP, Sankaran B, Surolia N, Surolia A, Suguna K IUBMB Life. 2010 Jun;62(6):467-76. PMID:20503440<ref>PMID:20503440</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3lt2" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20503440}}, adds the Publication Abstract to the page
+*[[Enoyl-Acyl-Carrier Protein Reductase 3D structures|Enoyl-Acyl-Carrier Protein Reductase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20503440 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20503440}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-LT2 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LT2 OCA].
-==Reference==
-<ref group="xtra">PMID:20503440</ref><references group="xtra"/>
 [[Category: Plasmodium falciparum]]
-[[Category: Bhargav, S P.]]
+[[Category: Bhargav SP]]
-[[Category: Maity, K.]]
+[[Category: Maity K]]
-[[Category: Suguna, K.]]
+[[Category: Suguna K]]
-[[Category: Surolia, A.]]
+[[Category: Surolia A]]
-[[Category: Surolia, N.]]
+[[Category: Surolia N]]
-[[Category: Enoyl-acp reductase]]
-[[Category: Oxidoreductase]]
-[[Category: P falciparum]]
-[[Category: Triclosan]]
-[[Category: Triclosan variant]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 16 08:40:50 2010''