3lk4: Difference between revisions

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-{{Seed}}
-[[Image:3lk4.jpg|left|200px]]
-<!--
+==Crystal structure of CapZ bound to the uncapping motif from CD2AP==
-The line below this paragraph, containing "STRUCTURE_3lk4", creates the "Structure Box" on the page.
+<StructureSection load='3lk4' size='340' side='right'caption='[[3lk4]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3lk4]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LK4 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lk4 OCA], [https://pdbe.org/3lk4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lk4 RCSB], [https://www.ebi.ac.uk/pdbsum/3lk4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lk4 ProSAT]</span></td></tr>
-{{STRUCTURE_3lk4|  PDB=3lk4  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CAZA1_CHICK CAZA1_CHICK] F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. CapZ may mediate the attachment of the barbed ends of actin filaments to the Z-line.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/3lk4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lk4 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Capping protein (CP) regulates actin dynamics by binding the barbed ends of actin filaments. Removal of CP may be one means to harness actin polymerization for processes such as cell movement and endocytosis. Here we structurally and biochemically investigated a CP interaction (CPI) motif present in the otherwise unrelated proteins CARMIL and CD2AP. The CPI motif wraps around the stalk of the mushroom-shaped CP at a site distant from the actin-binding interface, which lies on the top of the mushroom cap. We propose that the CPI motif may act as an allosteric modulator, restricting CP to a low-affinity, filament-binding conformation. Structure-based sequence alignments extend the CPI motif-containing family to include CIN85, CKIP-1, CapZIP and a relatively uncharacterized protein, WASHCAP (FAM21). Peptides comprising these CPI motifs are able to inhibit CP and to uncap CP-bound actin filaments.
-===Crystal structure of CapZ bound to the uncapping motif from CD2AP===
+Structural characterization of a capping protein interaction motif defines a family of actin filament regulators.,Hernandez-Valladares M, Kim T, Kannan B, Tung A, Aguda AH, Larsson M, Cooper JA, Robinson RC Nat Struct Mol Biol. 2010 Apr;17(4):497-503. Epub 2010 Mar 28. PMID:20357771<ref>PMID:20357771</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3lk4" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-LK4 is a 36 chains structure with sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LK4 OCA].
+*[[CD2-associated protein 3D structures|CD2-associated protein 3D structures]]
+*[[F-actin capping protein|F-actin capping protein]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Gallus gallus]]
-[[Category: Cooper, J A.]]
+[[Category: Homo sapiens]]
-[[Category: Hernandez-Valladares, M.]]
+[[Category: Large Structures]]
-[[Category: Kannan, B.]]
+[[Category: Cooper JA]]
-[[Category: Kim, T.]]
+[[Category: Hernandez-Valladares M]]
-[[Category: Robinson, R C.]]
+[[Category: Kannan B]]
-[[Category: Tung, A.]]
+[[Category: Kim T]]
-[[Category: Actin capping]]
+[[Category: Robinson RC]]
-[[Category: Actin filament]]
+[[Category: Tung A]]
-[[Category: Actin-binding]]
-[[Category: Actin-filament regulator]]
-[[Category: Capz]]
-[[Category: Cd2ap]]
-[[Category: Cytoplasm]]
-[[Category: Cytoskeleton]]
-[[Category: Protein binding]]
-[[Category: Protein-protein comple]]
-[[Category: Uncapping]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr  7 10:50:42 2010''