3irp: Difference between revisions

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[[Image:3irp.png|left|200px]]


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==Crystal structure of functional region of UafA from Staphylococcus saprophyticus at 1.50 angstrom resolution==
The line below this paragraph, containing "STRUCTURE_3irp", creates the "Structure Box" on the page.
<StructureSection load='3irp' size='340' side='right'caption='[[3irp]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3irp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_saprophyticus_subsp._saprophyticus_ATCC_15305_=_NCTC_7292 Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IRP FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
{{STRUCTURE_3irp|  PDB=3irp  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3irp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3irp OCA], [https://pdbe.org/3irp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3irp RCSB], [https://www.ebi.ac.uk/pdbsum/3irp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3irp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/UAFA_STAS1 UAFA_STAS1]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ir/3irp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3irp ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococci use cell wall-anchored proteins as adhesins to attach to host tissues. Staphylococcus saprophyticus, a uropathogenic species, has a unique cell wall-anchored protein, uro-adherence factor A (UafA), which shows erythrocyte binding activity. To investigate the mechanism of adhesion by UafA, we determined the crystal structure of the functional region of UafA at 1.5 A resolution. The structure was composed of three domains, designated as the N2, N3, and B domains, arranged in a triangular relative configuration. Hemagglutination inhibition assay with domain-truncated mutants indicated that both N and B domains were necessary for erythrocyte binding. Based on these results, a novel manner of ligand binding in which the B domain acts as a functional domain was proposed as the adhesion mechanism of S. saprophyticus.


===Crystal structure of functional region of UafA from Staphylococcus saprophyticus at 1.50 angstrom resolution===
Crystal structure of the functional region of Uro-adherence factor A from Staphylococcus saprophyticus reveals participation of the B domain in ligand binding.,Matsuoka E, Tanaka Y, Kuroda M, Shouji Y, Ohta T, Tanaka I, Yao M Protein Sci. 2011 Feb;20(2):406-16. doi: 10.1002/pro.573. PMID:21280131<ref>PMID:21280131</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
The line below this paragraph, {{ABSTRACT_PUBMED_21162026}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3irp" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 21162026 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_21162026}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3irp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_saprophyticus_subsp._saprophyticus Staphylococcus saprophyticus subsp. saprophyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IRP OCA].
[[Category: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292]]
 
[[Category: Kuroda M]]
==Reference==
[[Category: Matsuoka E]]
<ref group="xtra">PMID:21162026</ref><references group="xtra"/>
[[Category: Shouji Y]]
[[Category: Staphylococcus saprophyticus subsp. saprophyticus]]
[[Category: Tanaka I]]
[[Category: Kuroda, M.]]
[[Category: Tanaka Y]]
[[Category: Matsuoka, E.]]
[[Category: Yao M]]
[[Category: Shouji, Y.]]
[[Category: Tanaka, I.]]
[[Category: Tanaka, Y.]]
[[Category: Yao, M.]]

Latest revision as of 19:02, 1 November 2023

Crystal structure of functional region of UafA from Staphylococcus saprophyticus at 1.50 angstrom resolutionCrystal structure of functional region of UafA from Staphylococcus saprophyticus at 1.50 angstrom resolution

Structural highlights

3irp is a 1 chain structure with sequence from Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UAFA_STAS1

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Staphylococci use cell wall-anchored proteins as adhesins to attach to host tissues. Staphylococcus saprophyticus, a uropathogenic species, has a unique cell wall-anchored protein, uro-adherence factor A (UafA), which shows erythrocyte binding activity. To investigate the mechanism of adhesion by UafA, we determined the crystal structure of the functional region of UafA at 1.5 A resolution. The structure was composed of three domains, designated as the N2, N3, and B domains, arranged in a triangular relative configuration. Hemagglutination inhibition assay with domain-truncated mutants indicated that both N and B domains were necessary for erythrocyte binding. Based on these results, a novel manner of ligand binding in which the B domain acts as a functional domain was proposed as the adhesion mechanism of S. saprophyticus.

Crystal structure of the functional region of Uro-adherence factor A from Staphylococcus saprophyticus reveals participation of the B domain in ligand binding.,Matsuoka E, Tanaka Y, Kuroda M, Shouji Y, Ohta T, Tanaka I, Yao M Protein Sci. 2011 Feb;20(2):406-16. doi: 10.1002/pro.573. PMID:21280131[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Matsuoka E, Tanaka Y, Kuroda M, Shouji Y, Ohta T, Tanaka I, Yao M. Crystal structure of the functional region of Uro-adherence factor A from Staphylococcus saprophyticus reveals participation of the B domain in ligand binding. Protein Sci. 2011 Feb;20(2):406-16. doi: 10.1002/pro.573. PMID:21280131 doi:http://dx.doi.org/10.1002/pro.573

3irp, resolution 1.50Å

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