3ii3: Difference between revisions
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< | ==Structure of ORF157 from Acidianus filamentous Virus 1== | ||
<StructureSection load='3ii3' size='340' side='right'caption='[[3ii3]], [[Resolution|resolution]] 2.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3ii3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidianus_filamentous_virus_1 Acidianus filamentous virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3II3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3II3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | |||
-- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ii3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ii3 OCA], [https://pdbe.org/3ii3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ii3 RCSB], [https://www.ebi.ac.uk/pdbsum/3ii3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ii3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Y157_AFV1Y Y157_AFV1Y] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Acidianus filamentous virus 1 (AFV1) (Lipothrixviridae) is an enveloped filamentous virus that was characterized from a crenarchaeal host. It infects Acidianus species that thrive in the acidic hot springs (>85 degrees C and pH <3) of Yellowstone National Park, WY. The AFV1 20.8-kb, linear, double-stranded DNA genome encodes 40 putative open reading frames whose sequences generally show little similarity to other genes in the sequence databases. Because three-dimensional structures are more conserved than sequences and hence are more effective at revealing function, we set out to determine protein structures from putative AFV1 open reading frames (ORF). The crystal structure of ORF157 reveals an alpha+beta protein with a novel fold that remotely resembles the nucleotidyltransferase topology. In vitro, AFV1-157 displays a nuclease activity on linear double-stranded DNA. Alanine substitution mutations demonstrated that E86 is essential to catalysis. AFV1-157 represents a novel class of nuclease, but its exact role in vivo remains to be determined. | |||
ORF157 from the archaeal virus Acidianus filamentous virus 1 defines a new class of nuclease.,Goulet A, Pina M, Redder P, Prangishvili D, Vera L, Lichiere J, Leulliot N, van Tilbeurgh H, Ortiz-Lombardia M, Campanacci V, Cambillau C J Virol. 2010 May;84(10):5025-31. Epub 2010 Mar 3. PMID:20200253<ref>PMID:20200253</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3ii3" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
== | |||
< | |||
[[Category: Acidianus filamentous virus 1]] | [[Category: Acidianus filamentous virus 1]] | ||
[[Category: Cambillau | [[Category: Large Structures]] | ||
[[Category: Campanacci | [[Category: Cambillau C]] | ||
[[Category: Goulet | [[Category: Campanacci V]] | ||
[[Category: Pina | [[Category: Goulet A]] | ||
[[Category: Prangishvili | [[Category: Pina M]] | ||
[[Category: Redder | [[Category: Prangishvili D]] | ||
[[Category: Tilbeurgh | [[Category: Redder P]] | ||
[[Category: Van Tilbeurgh H]] | |||
Latest revision as of 18:58, 1 November 2023
Structure of ORF157 from Acidianus filamentous Virus 1Structure of ORF157 from Acidianus filamentous Virus 1
Structural highlights
FunctionPublication Abstract from PubMedAcidianus filamentous virus 1 (AFV1) (Lipothrixviridae) is an enveloped filamentous virus that was characterized from a crenarchaeal host. It infects Acidianus species that thrive in the acidic hot springs (>85 degrees C and pH <3) of Yellowstone National Park, WY. The AFV1 20.8-kb, linear, double-stranded DNA genome encodes 40 putative open reading frames whose sequences generally show little similarity to other genes in the sequence databases. Because three-dimensional structures are more conserved than sequences and hence are more effective at revealing function, we set out to determine protein structures from putative AFV1 open reading frames (ORF). The crystal structure of ORF157 reveals an alpha+beta protein with a novel fold that remotely resembles the nucleotidyltransferase topology. In vitro, AFV1-157 displays a nuclease activity on linear double-stranded DNA. Alanine substitution mutations demonstrated that E86 is essential to catalysis. AFV1-157 represents a novel class of nuclease, but its exact role in vivo remains to be determined. ORF157 from the archaeal virus Acidianus filamentous virus 1 defines a new class of nuclease.,Goulet A, Pina M, Redder P, Prangishvili D, Vera L, Lichiere J, Leulliot N, van Tilbeurgh H, Ortiz-Lombardia M, Campanacci V, Cambillau C J Virol. 2010 May;84(10):5025-31. Epub 2010 Mar 3. PMID:20200253[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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