3h4t: Difference between revisions

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-[[Image:3h4t.png|left|200px]]
-<!--
+==Chimeric Glycosyltransferase for the generation of novel natural products - GtfAH1 in complex with UDP-2F-Glc==
-The line below this paragraph, containing "STRUCTURE_3h4t", creates the "Structure Box" on the page.
+<StructureSection load='3h4t' size='340' side='right'caption='[[3h4t]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3h4t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinoplanes_teichomyceticus Actinoplanes teichomyceticus] and [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis Amycolatopsis orientalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H4T FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-{{STRUCTURE_3h4t|  PDB=3h4t  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4t OCA], [https://pdbe.org/3h4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h4t RCSB], [https://www.ebi.ac.uk/pdbsum/3h4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h4t ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GTFA_AMYOR GTFA_AMYOR] Catalyzes the attachment of 4-epi-vancosamine from a TDP donor to the beta-OH-Tyr-6 of the aglycone cosubstrate in the biosynthesis of glycopeptide antibiotic chloroeremomycin, a member of the vancomycin group of antibiotics. Strongly prefers devancoaminyl-vancomycin (DVV) as substrate rather than the heptapeptide vancomycin aglycone (AGV). Acts downstream of GtfB.<ref>PMID:15070728</ref> <ref>PMID:19549605</ref> <ref>PMID:9115410</ref> [https://www.uniprot.org/uniprot/Q6ZZJ7_ACTTI Q6ZZJ7_ACTTI]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/3h4t_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h4t ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Glycodiversification, an invaluable tool for generating biochemical diversity, can be catalyzed by glycosyltransferases, which attach activated sugar "donors" onto "acceptor" molecules. However, many glycosyltransferases can tolerate only minor modifications to their native substrates, thus making them unsuitable tools for current glycodiversification strategies. Here we report the production of functional chimeric glycosyltransferases by mixing and matching the N- and C-terminal domains of glycopeptide glycosyltransferases. Using this method we have generated hybrid glycopeptides and have demonstrated that domain swapping can result in a predictable switch of substrate specificity, illustrating that N- and C-terminal domains predominantly dictate acceptor and donor specificity, respectively. The determination of the structure of a chimera in complex with a sugar donor analog shows that almost all sugar-glycosyltransferase binding interactions occur in the C-terminal domain.
-===Chimeric Glycosyltransferase for the generation of novel natural products - GtfAH1 in complex with UDP-2F-Glc===
+Chimeric glycosyltransferases for the generation of hybrid glycopeptides.,Truman AW, Dias MV, Wu S, Blundell TL, Huang F, Spencer JB Chem Biol. 2009 Jun 26;16(6):676-85. PMID:19549605<ref>PMID:19549605</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3h4t" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_19549605}}, adds the Publication Abstract to the page
+*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 19549605 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_19549605}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Actinoplanes teichomyceticus]]
-[[3h4t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amycolatopsis_orientalis,_actinoplanes_teichomyceticus Amycolatopsis orientalis, actinoplanes teichomyceticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4T OCA].
+[[Category: Amycolatopsis orientalis]]
+[[Category: Large Structures]]
-==Reference==
+[[Category: Blundell TL]]
-<ref group="xtra">PMID:019549605</ref><references group="xtra"/>
+[[Category: Dias MVB]]
-[[Category: Amycolatopsis orientalis, actinoplanes teichomyceticus]]
+[[Category: Huang F]]
-[[Category: Blundell, T L.]]
+[[Category: Spencer JB]]
-[[Category: Dias, M V.B.]]
+[[Category: Truman AW]]
-[[Category: Huang, F.]]
+[[Category: Wu S]]
-[[Category: Spencer, J B.]]
-[[Category: Truman, A W.]]
-[[Category: Wu, S.]]
-[[Category: Antibiotic]]
-[[Category: Glycosyltransferase]]
-[[Category: Gtfa]]
-[[Category: Natural product]]
-[[Category: Orf1]]
-[[Category: Teicoplanin]]
-[[Category: Vancomycin]]