3fht: Difference between revisions

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-[[Image:3fht.png|left|200px]]
-{{STRUCTURE_3fht|  PDB=3fht  |  SCENE=  }}
+==Crystal structure of human Dbp5 in complex with AMPPNP and RNA==
+<StructureSection load='3fht' size='340' side='right'caption='[[3fht]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3fht]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FHT FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fht OCA], [https://pdbe.org/3fht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fht RCSB], [https://www.ebi.ac.uk/pdbsum/3fht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fht ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DD19B_HUMAN DD19B_HUMAN] ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/3fht_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fht ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The DEAD-box protein DBP5 is essential for mRNA export in both yeast and humans. It binds RNA and is concentrated and locally activated at the cytoplasmic side of the nuclear pore complex. We have determined the crystal structures of human DBP5 bound to RNA and AMPPNP, and bound to the cytoplasmic nucleoporin NUP214. The structures reveal that binding of DBP5 to nucleic acid and to NUP214 is mutually exclusive. Using in vitro assays, we demonstrate that NUP214 decreases both the RNA binding and ATPase activities of DBP5. The interactions are mediated by conserved residues, implying a conserved recognition mechanism. These results suggest a framework for the consecutive steps leading to the release of mRNA at the final stages of nuclear export. More generally, they provide a paradigm for how binding of regulators can specifically inhibit DEAD-box proteins.
-===Crystal structure of human Dbp5 in complex with AMPPNP and RNA===
+The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner.,von Moeller H, Basquin C, Conti E Nat Struct Mol Biol. 2009 Feb 15. PMID:19219046<ref>PMID:19219046</ref>
-{{ABSTRACT_PUBMED_19219046}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 3fht" style="background-color:#fffaf0;"></div>
-[[3fht]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FHT OCA].
 ==See Also==
-*[[Helicase|Helicase]]
+*[[Helicase 3D structures|Helicase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019219046</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Conti, E.]]
+[[Category: Large Structures]]
-[[Category: Moeller, H von.]]
+[[Category: Conti E]]
-[[Category: Atp-binding]]
+[[Category: Von Moeller H]]
-[[Category: Can]]
-[[Category: Dbp5]]
-[[Category: Ddx19b]]
-[[Category: Dead-box helicase]]
-[[Category: Gle1]]
-[[Category: Helicase]]
-[[Category: Hydrolase]]
-[[Category: Hydrolase-rna complex]]
-[[Category: Membrane]]
-[[Category: Mrna export]]
-[[Category: Mrna transport]]
-[[Category: Nuclear pore]]
-[[Category: Nuclear pore complex]]
-[[Category: Nucleocytoplasmic transport]]
-[[Category: Nucleotide-binding]]
-[[Category: Nucleus]]
-[[Category: Nup159]]
-[[Category: Nup214]]
-[[Category: Phosphoprotein]]
-[[Category: Protein transport]]
-[[Category: Rna dependent atpase]]
-[[Category: Rna-binding]]
-[[Category: Translocation]]
-[[Category: Transport]]