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<StructureSection load='3d4x' size='340' side='right'caption='[[3d4x]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='3d4x' size='340' side='right'caption='[[3d4x]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3d4x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Felis_silvestris_catus Felis silvestris catus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D4X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3D4X FirstGlance]. <br>
<table><tr><td colspan='2'>[[3d4x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Felis_catus Felis catus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D4X FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qu0|2qu0]], [[2ri4|2ri4]], [[3cy5|3cy5]], [[3d1a|3d1a]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d4x OCA], [http://pdbe.org/3d4x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d4x RCSB], [http://www.ebi.ac.uk/pdbsum/3d4x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3d4x ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d4x OCA], [https://pdbe.org/3d4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d4x RCSB], [https://www.ebi.ac.uk/pdbsum/3d4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d4x ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/HBA_FELCA HBA_FELCA]] Involved in oxygen transport from the lung to the various peripheral tissues. [[http://www.uniprot.org/uniprot/HBB_FELCA HBB_FELCA]] Involved in oxygen transport from the lung to the various peripheral tissues.  
[https://www.uniprot.org/uniprot/HBB_FELCA HBB_FELCA] Involved in oxygen transport from the lung to the various peripheral tissues.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d4x ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d4x ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Hemoglobin is a tetrameric, iron-containing metalloprotein, which plays a vital role in the transportation of oxygen from lungs to tissues and carbon dioxide back to lungs. Though good amount of work has already been done on hemoglobins, the scarcity of data on three dimensional structures pertaining to low oxygen affinity hemoglobins from mammalian species, motivated our group to work on this problem specifically. Herein, we report the preliminary crystallographic analysis of buffalo hemoglobin, which belongs to low oxygen affinity species. The buffalo blood was collected, purified by anion exchange chromatography and crystallized with PEG 3350 using 50mM phosphate buffer at pH 6.7 as a precipitant by hanging drop vapor diffusion method. Data collection was carried out using mar345dtb image plate detector system. Buffalo hemoglobin crystallizes in orthorhombic space group P2(1)2(1)2(1) with one whole biological molecule (alpha2beta2) in the asymmetric unit with cell dimensions a=63.064A, b=74.677A, c=110.224A.
Preliminary Crystallographic Study of Hemoglobin from Buffalo (Bubalus bubalis): A Low Oxygen Affinity Species.,Balasubramanian M, Moorthy PS, Neelagandan K, Ponnuswamy MN Protein Pept Lett. 2009;16(2):213-5. PMID:19200047<ref>PMID:19200047</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3d4x" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Felis silvestris catus]]
[[Category: Felis catus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Balasubramanian, M]]
[[Category: Balasubramanian M]]
[[Category: Moorthy, P Sathya]]
[[Category: Neelagandan K]]
[[Category: Neelagandan, K]]
[[Category: Ponnuswamy MN]]
[[Category: Ponnuswamy, M N]]
[[Category: Sathya Moorthy P]]
[[Category: Allosteric mechanism]]
[[Category: Heme]]
[[Category: Hemoglobin]]
[[Category: Iron]]
[[Category: Low oxygen affinity]]
[[Category: Monoclinic]]
[[Category: Oxygen storage]]
[[Category: Oxygen transport]]
[[Category: Transport]]

Latest revision as of 18:03, 1 November 2023

Crystal structure determination of cat (Felis silvestris catus) hemoglobin at 2.2 angstrom resolutionCrystal structure determination of cat (Felis silvestris catus) hemoglobin at 2.2 angstrom resolution

Structural highlights

3d4x is a 4 chain structure with sequence from Felis catus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HBB_FELCA Involved in oxygen transport from the lung to the various peripheral tissues.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

3d4x, resolution 2.20Å

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OCA
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