3bsx: Difference between revisions
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<StructureSection load='3bsx' size='340' side='right'caption='[[3bsx]], [[Resolution|resolution]] 2.32Å' scene=''> | <StructureSection load='3bsx' size='340' side='right'caption='[[3bsx]], [[Resolution|resolution]] 2.32Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3bsx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3bsx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BSX FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsx OCA], [https://pdbe.org/3bsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsx RCSB], [https://www.ebi.ac.uk/pdbsum/3bsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsx ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsx OCA], [https://pdbe.org/3bsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsx RCSB], [https://www.ebi.ac.uk/pdbsum/3bsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsx ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/PUM1_HUMAN PUM1_HUMAN] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity). | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Aggarwal | [[Category: Aggarwal AK]] | ||
[[Category: Gupta | [[Category: Gupta YK]] | ||
[[Category: Nair | [[Category: Nair DT]] | ||
[[Category: Wharton | [[Category: Wharton RP]] | ||
Latest revision as of 17:50, 1 November 2023
Crystal Structure of Human Pumilio 1 in complex with Puf5 RNACrystal Structure of Human Pumilio 1 in complex with Puf5 RNA
Structural highlights
FunctionPUM1_HUMAN Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedPumilio is a founder member of the evolutionarily conserved Puf family of RNA-binding proteins that control a number of physiological processes in eukaryotes. A structure of human Pumilio (hPum) Puf domain bound to a Drosophila regulatory sequence showed that each Puf repeat recognizes a single nucleotide. Puf domains in general bind promiscuously to a large set of degenerate sequences, but the structural basis for this promiscuity has been unclear. Here, we describe the structures of hPum Puf domain complexed to two noncognate RNAs, CycB(reverse) and Puf5. In each complex, one of the nucleotides is ejected from the binding surface, in effect, acting as a "spacer." The complexes also reveal the plasticity of several Puf repeats, which recognize noncanonical nucleotides. Together, these complexes provide a molecular basis for recognition of degenerate binding sites, which significantly increases the number of mRNAs targeted for regulation by Puf proteins in vivo. Structures of human Pumilio with noncognate RNAs reveal molecular mechanisms for binding promiscuity.,Gupta YK, Nair DT, Wharton RP, Aggarwal AK Structure. 2008 Apr;16(4):549-57. Epub 2008 Mar 6. PMID:18328718[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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