3b99: Difference between revisions
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==Crystal structure of zebrafish prostacyclin synthase (cytochrome P450 8A1) in complex with substrate analog U51605== | ==Crystal structure of zebrafish prostacyclin synthase (cytochrome P450 8A1) in complex with substrate analog U51605== | ||
<StructureSection load='3b99' size='340' side='right' caption='[[3b99]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='3b99' size='340' side='right'caption='[[3b99]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3b99]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3b99]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B99 FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=U51:(5Z)-7-{(1R,4S,5R,6R)-6-[(1E)-OCT-1-EN-1-YL]-2,3-DIAZABICYCLO[2.2.1]HEPT-2-EN-5-YL}HEPT-5-ENOIC+ACID'>U51</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=U51:(5Z)-7-{(1R,4S,5R,6R)-6-[(1E)-OCT-1-EN-1-YL]-2,3-DIAZABICYCLO[2.2.1]HEPT-2-EN-5-YL}HEPT-5-ENOIC+ACID'>U51</scene></td></tr> | |||
<tr | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b99 OCA], [https://pdbe.org/3b99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b99 RCSB], [https://www.ebi.ac.uk/pdbsum/3b99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b99 ProSAT]</span></td></tr> | ||
</table> | |||
== Function == | |||
<table> | [https://www.uniprot.org/uniprot/PTGIS_DANRE PTGIS_DANRE] Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).<ref>PMID:18032380</ref> Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation (PubMed:18032380). Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) (By similarity).[UniProtKB:Q16647]<ref>PMID:18032380</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/3b99_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/3b99_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b99 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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Structures of prostacyclin synthase and its complexes with substrate analog and inhibitor reveal a ligand-specific heme conformation change.,Li YC, Chiang CW, Yeh HC, Hsu PY, Whitby FG, Wang LH, Chan NL J Biol Chem. 2008 Feb 1;283(5):2917-26. Epub 2007 Nov 21. PMID:18032380<ref>PMID:18032380</ref> | Structures of prostacyclin synthase and its complexes with substrate analog and inhibitor reveal a ligand-specific heme conformation change.,Li YC, Chiang CW, Yeh HC, Hsu PY, Whitby FG, Wang LH, Chan NL J Biol Chem. 2008 Feb 1;283(5):2917-26. Epub 2007 Nov 21. PMID:18032380<ref>PMID:18032380</ref> | ||
From | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3b99" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Danio rerio]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Chan | [[Category: Chan N-L]] | ||
[[Category: Chiang | [[Category: Chiang C-W]] | ||
[[Category: Hsu | [[Category: Hsu P-Y]] | ||
[[Category: Li | [[Category: Li Y-C]] | ||
[[Category: Wang | [[Category: Wang L-H]] | ||
[[Category: Whitby | [[Category: Whitby FG]] | ||
[[Category: Yeh | [[Category: Yeh H-C]] | ||