3a8p: Difference between revisions

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-{{Seed}}
-[[Image:3a8p.png|left|200px]]
-<!--
+==Crystal structure of the Tiam2 PHCCEx domain==
-The line below this paragraph, containing "STRUCTURE_3a8p", creates the "Structure Box" on the page.
+<StructureSection load='3a8p' size='340' side='right'caption='[[3a8p]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3a8p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A8P FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a8p OCA], [https://pdbe.org/3a8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a8p RCSB], [https://www.ebi.ac.uk/pdbsum/3a8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a8p ProSAT]</span></td></tr>
-{{STRUCTURE_3a8p|  PDB=3a8p  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TIAM2_MOUSE TIAM2_MOUSE] Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates specifically RAC1, but not CDC42 and RHOA. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane.<ref>PMID:10364228</ref> <ref>PMID:11707441</ref> <ref>PMID:12912917</ref> <ref>PMID:14550769</ref> <ref>PMID:17320046</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/3a8p_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a8p ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Tiam1 and Tiam2 (Tiam1/2) are guanine nucleotide-exchange factors that possess the PH-CC-Ex (pleckstrin homology, coiled coil and extra) region that mediates binding to plasma membranes and signalling proteins in the activation of Rac GTPases. Crystal structures of the PH-CC-Ex regions revealed a single globular domain, PHCCEx domain, comprising a conventional PH subdomain associated with an antiparallel coiled coil of CC subdomain and a novel three-helical globular Ex subdomain. The PH subdomain resembles the beta-spectrin PH domain, suggesting non-canonical phosphatidylinositol binding. Mutational and binding studies indicated that CC and Ex subdomains form a positively charged surface for protein binding. We identified two unique acidic sequence motifs in Tiam1/2-interacting proteins for binding to PHCCEx domain, Motif-I in CD44 and ephrinB's and the NMDA receptor, and Motif-II in Par3 and JIP2. Our results suggest the molecular basis by which the Tiam1/2 PHCCEx domain facilitates dual binding to membranes and signalling proteins.
-===Crystal structure of the Tiam2 PHCCEx domain===
+The PHCCEx domain of Tiam1/2 is a novel protein- and membrane-binding module.,Terawaki S, Kitano K, Mori T, Zhai Y, Higuchi Y, Itoh N, Watanabe T, Kaibuchi K, Hakoshima T EMBO J. 2010 Jan 6;29(1):236-50. Epub 2009 Nov 5. PMID:19893486<ref>PMID:19893486</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_19893486}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3a8p" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 19893486 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_19893486}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-A8P is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A8P OCA].
-==Reference==
-<ref group="xtra">PMID:19893486</ref><references group="xtra"/>
 [[Category: Mus musculus]]
-[[Category: Hakoshima, T.]]
+[[Category: Hakoshima T]]
-[[Category: Higuchi, Y.]]
+[[Category: Higuchi Y]]
-[[Category: Itoh, N.]]
+[[Category: Itoh N]]
-[[Category: Kaibuchi, K.]]
+[[Category: Kaibuchi K]]
-[[Category: Kitano, K.]]
+[[Category: Kitano K]]
-[[Category: Mori, T.]]
+[[Category: Mori T]]
-[[Category: Terawaki, S.]]
+[[Category: Terawaki S]]
-[[Category: Watanabe, T.]]
+[[Category: Watanabe T]]
-[[Category: Zhai, Y.]]
+[[Category: Zhai Y]]
-[[Category: Alternative splicing]]
-[[Category: Cell projection]]
-[[Category: Coiled coil]]
-[[Category: Cytoplasm]]
-[[Category: Guanine nucleotide exchange factor]]
-[[Category: Guanine-nucleotide releasing factor]]
-[[Category: Lipoprotein]]
-[[Category: Myristate]]
-[[Category: Phosphoprotein]]
-[[Category: Signaling protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 20 16:22:22 2010''