2zb8: Difference between revisions

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-{{Seed}}
-[[Image:2zb8.png|left|200px]]
-<!--
+==Crystal structure of human 15-ketoprostaglandin delta-13-reductase in complex with NADP and indomethacin==
-The line below this paragraph, containing "STRUCTURE_2zb8", creates the "Structure Box" on the page.
+<StructureSection load='2zb8' size='340' side='right'caption='[[2zb8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2zb8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZB8 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMN:INDOMETHACIN'>IMN</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_2zb8|  PDB=2zb8  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zb8 OCA], [https://pdbe.org/2zb8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zb8 RCSB], [https://www.ebi.ac.uk/pdbsum/2zb8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zb8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PTGR2_HUMAN PTGR2_HUMAN] Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation (By similarity).<ref>PMID:19000823</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/2zb8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zb8 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+PTGR2 catalyzes an NADPH-dependent reduction of the conjugated alpha,beta-unsaturated double bond of 15-keto-PGE(2), a key step in terminal inactivation of prostaglandins and suppression of PPARgamma-mediated adipocyte differentiation. Selective inhibition of PTGR2 may contribute to the improvement of insulin sensitivity with fewer side effects. PTGR2 belongs to the medium-chain dehydrogenase/reductase superfamily. The crystal structures reported here reveal features of the NADPH binding-induced conformational change in a LID motif and a polyproline type II helix which are critical for the reaction. Mutation of Tyr64 and Tyr259 significantly reduces the rate of catalysis but increases the affinity to substrate, confirming the structural observations. Besides targeting cyclooxygenase, indomethacin also inhibits PTGR2 with a binding mode similar to that of 15-keto-PGE(2). The LID motif becomes highly disordered upon the binding of indomethacin, indicating plasticity of the active site. This study has implications for the rational design of inhibitors of PTGR2.
-===Crystal structure of human 15-ketoprostaglandin delta-13-reductase in complex with NADP and indomethacin===
+Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2.,Wu YH, Ko TP, Guo RT, Hu SM, Chuang LM, Wang AH Structure. 2008 Nov 12;16(11):1714-23. PMID:19000823<ref>PMID:19000823</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_19000823}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2zb8" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 19000823 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_19000823}}
+__TOC__
+</StructureSection>
-==About this Structure==
-ZB8 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZB8 OCA].
-==Reference==
-Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2., Wu YH, Ko TP, Guo RT, Hu SM, Chuang LM, Wang AH, Structure. 2008 Nov 12;16(11):1714-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19000823 19000823]
-[[Category: 15-oxoprostaglandin 13-oxidase]]
 [[Category: Homo sapiens]]
-[[Category: Chuang, L M.]]
+[[Category: Large Structures]]
-[[Category: Guo, R T.]]
+[[Category: Chuang LM]]
-[[Category: Hu, S M.]]
+[[Category: Guo RT]]
-[[Category: Ko, T P.]]
+[[Category: Hu SM]]
-[[Category: Wang, A H.J.]]
+[[Category: Ko TP]]
-[[Category: Wu, Y H.]]
+[[Category: Wang AHJ]]
-[[Category: Alternative splicing]]
+[[Category: Wu YH]]
-[[Category: Cytoplasm]]
-[[Category: Nadp]]
-[[Category: Oxidoreductase]]
-[[Category: Rossmann fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 24 10:56:44 2008''