Ribosome inactivating protein: Difference between revisions

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<StructureSection load='' size='350' side='right' caption='Ribosome inactivating protein from pokeweed complex with adenine (PDB code [[1qci]])' scene='70/706276/Cv/1'>
<StructureSection load='' size='350' side='right' caption='Ribosome inactivating protein from pokeweed complex with adenine (PDB code [[1qci]])' scene='70/706276/Cv/1'>
== Function ==
== Function ==
'''Ribosome inactivating protein''' (RIP) is a bacterial or plant toxin which inhibits protein synthesis.  RIP inactivates the ribosomal 60S subunit by N-glycosidic cleavage which results in releasing a specific adenine from 28S rRNA<ref>PMID:3510899</ref>.  '''Type 1 RIP''' have a single chain, while '''type 2 RIP''' (i.e., ricin, ebulin and nigrin) have two chains.  '''Momorcharin''' is a RIP isolated from several plants<ref>PMID:23777339</ref>.
'''Ribosome inactivating protein''' (RIP) is a bacterial or plant toxin which inhibits protein synthesis.  RIP inactivates the ribosomal 60S subunit by N-glycosidic cleavage which results in releasing a specific adenine from 28S rRNA<ref>PMID:3510899</ref>.  '''Type 1 RIP''' have a single chain, while '''type 2 RIP''' (i.e., ricin, ebulin and nigrin) have two chains.  '''Momorcharin''' is a RIP isolated from several plants<ref>PMID:23777339</ref>. '''Trichosanthin''' is a RIP isolated from snake gourd<ref>PMID:15804517</ref>.  


== Relevance ==
== Relevance ==
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== 3D Structures of ribosome inactivating protein ==
== 3D Structures of ribosome inactivating protein ==
[[Ribosome inactivating protein 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|
*Pokeweed ribosome inactivating protein
**[[1apa]], [[1paf]], [[1pag]], [[1qcg]] – apRIP – american pokeweed <br />
**[[1j1q]], [[1gik]] – apRIP-S<br />
**[[1j1r]] – apRIP-S + adenine<br />
**[[1j1s]] – apRIP-S + formycin<br />
**[[1qci]] – apRIP + adenine<br />
**[[1d6a]] – apRIP + guanine<br />
**[[1qcj]] – apRIP + pteoric acid<br />
**[[1lln]] – apRIP-3 + methylated lysine<br />
*Phytolacca dioica ribosome inactivating protein
**[[3h5k]] – PdRIP PD-L1 – ''Phytolacca dioica''<br />
**[[3le7]] – PdRIP PD-L1 + adenine <br />
**[[2z4u]] – PdRIP PD-L4 <br />
**[[2z53]] – PdRIP PD-L4 (mutant) <br />
**[[2qes]] – PdRIP PD-L4 + adenine <br />
**[[2qet]] – PdRIP PD-L4 (mutant) + adenine<br />
*Dianthin 30 ribosome inactivating protein
**[[1lp8]], [[1rl0]] – cpRIP – clove pink<br />
**[[1lpc]] – cpRIP + cAMP<br />
**[[1lpd]] – cpRIP + adenine<br />
*Type 1 ribosome inactivating protein
**[[2g5x]] – RIP – scarlet lychnis<br />
**[[3ctk]] – RIP – bougainvillea<br />
**[[3mrw]], [[3s9q]], [[4kmk]], [[4kwn]], [[4l66]], [[1cf5]], [[5gm7]], [[4rzj]] – bgRIP – bitter gourd<br />
**[[1f8q]], [[1aha]], [[1ahb]], [[1ahc]], [[1mri]] – bpα-RIP – balsam pear <br />
**[[3mvg]] – RIP – dutch iris <br />
*Type 1 ribosome inactivating protein complex with nucleotide
**[[3mry]] – bgRIP + aminopurine<br />
**[[4kpv]], [[5y48]] – bgRIP + pyrimidine derivative<br />
**[[3rl9]], [[3u6z]], [[3u70]], [[3v2k]] – bgRIP + adenine<br />
**[[4dwm]], [[4rzj]] – bgRIP + NAG<br />
**[[3n1n]], [[3my6]] – baRIP + guanine – balsam apple <br />
**[[4f9n]], [[4i47]] – bgRIP + guanine<br />
**[[5ilx]], [[4o0o]] – bgRIP + uracil derivative<br />
**[[5cso]], [[5cst]] – bgRIP + cytidine<br />
**[[4zt8]] – bgRIP + cytosine<br />
**[[4zu0]], [[4zz6]], [[4z44]], [[4o4q]], [[4o8e]], [[4q9f]], [[1mrg]], [[3q4p]], [[3qji]], [[4emf]], [[4emr]], [[5cso]], [[5cst]], [[5ilw]], [[5ilx]] – bgα-RIP + nucleotide <br />
*Type 1 ribosome inactivating protein complex with peptide
**[[4fxa]], [[4lt4]], [[4m5a]] – bgRIP + arginine derivative<br />
**[[3n3x]] – baRIP + polypeptide <br />
**[[3n2d]] – bgRIP + polypeptide <br />
*Type 1 ribosome inactivating protein other complexes
**[[3n31]], [[3n5d]], [[3n31]], [[3nfm]], [[3njs]], [[3nx9]], [[3n1d]] – baRIP + sugar <br />
**[[3v14]], [[4fz9]], [[4guw]], [[4hoa]], [[5wv1]] – bgRIP + sugar <br />
**[[4lro]] – bgRIP + spermidine<br />
**[[5y48]] – bgRIP + pyrimidine derivative<br />
**[[3sj6]] – bgRIP + oxalane derivative<br />
**[[3u6t]] – bgRIP + kanamycin<br />
**[[3u8f]] – bgRIP + mycolic acid<br />
**[[4h0z]] – bgRIP + acetyl-muramic acid<br />
**[[4jtp]] – bgRIP + ascorbic acid<br />
**[[4k2z]] – bgRIP + methylethylamine<br />
**[[5cix]] – bgRIP + triethanolamine<br />
**[[5gz7]] – bgRIP + PEG<br />
**[[4k2z]] – bgRIP + ethanediol<br />
**[[4jtb]] – bgRIP + phosphate<br />
**[[4lwx]] – bgRIP + peptidoglycan<br />
**[[4yp2]] – bgRIP + nicotinamide<br />
**[[1mrh]] – bpα-RIP + formycin<br />
**[[4fz9]], [[4xy7]] – bgα-RIP + disaccharide <br />
**[[5cf9]] – bgα-RIP + NADP <br />
*Type 2 ribosome inactivating protein
**[[5y42]] – angRIP – snake gourd<br />
**[[2jjr]], [[2vs6]] – sngRIP trichosanthin (mutant) <br />
**[[2mll]] – RIP – mistletoe<br />
**[[2vlc]] – RIP cinnamomin – camphor tree<br />
*Ribosome inactivating protein
**[[2k6h]] – cRIP – corn - NMR<br />
**[[2pqg]], [[2pqi]] – cRIP3 <br />
**[[2pqj]] – cRIP3 + adenine<br />
**[[2oqa]] – sgRIP – sponge gourd <br />
**[[2l37]], [[6o3s]] – sgRIP - NMR<br />
**[[3ktz]] – GmRIP gelonin – ''Gelonium multiforum''<br />
**[[3ku0]] – GmRIP gelonin + adenine<br />
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 12:15, 31 October 2023

Function

Ribosome inactivating protein (RIP) is a bacterial or plant toxin which inhibits protein synthesis. RIP inactivates the ribosomal 60S subunit by N-glycosidic cleavage which results in releasing a specific adenine from 28S rRNA[1]. Type 1 RIP have a single chain, while type 2 RIP (i.e., ricin, ebulin and nigrin) have two chains. Momorcharin is a RIP isolated from several plants[2]. Trichosanthin is a RIP isolated from snake gourd[3].

Relevance

RIP is being investigated as an immunotoxin for cancer treatment. Besides the anti-tumor activity of RIP, it was shown to have antiviral, antifungal, insecticidal, ribonuclease and deoxyribonuclease activities. Enhanced expression of RIP in plants confers an increased resistance to virus, fungi, insects, drought and salinity. α-momorcharin has potent inhibitory effect on some cancer cells[4].

Structural highlights

The [5]. Water molecules are shown as red spheres.

Ribosome inactivating protein from pokeweed complex with adenine (PDB code 1qci)

Drag the structure with the mouse to rotate

3D Structures of ribosome inactivating protein3D Structures of ribosome inactivating protein

Ribosome inactivating protein 3D structures

ReferencesReferences

  1. ↑ Stirpe F, Barbieri L. Ribosome-inactivating proteins up to date. FEBS Lett. 1986 Jan 20;195(1-2):1-8. PMID:3510899
  2. ↑ Wang S, Zheng Y, Yan J, Zhu Z, Wu Z, Ding Y. Alpha-momorcharin: a ribosome-inactivating protein from Momordica charantia, possessing DNA cleavage properties. Protein Pept Lett. 2013 Nov;20(11):1257-63. PMID:23777339
  3. ↑ Shaw PC, Lee KM, Wong KB. Recent advances in trichosanthin, a ribosome-inactivating protein with multiple pharmacological properties. Toxicon. 2005 May;45(6):683-9. PMID:15804517 doi:10.1016/j.toxicon.2004.12.024
  4. ↑ Ng TB, Liu WK, Sze SF, Yeung HW. Action of alpha-momorcharin, a ribosome inactivating protein, on cultured tumor cell lines. Gen Pharmacol. 1994 Jan;25(1):75-7. PMID:8026716
  5. ↑ Kurinov IV, Myers DE, Irvin JD, Uckun FM. X-ray crystallographic analysis of the structural basis for the interactions of pokeweed antiviral protein with its active site inhibitor and ribosomal RNA substrate analogs. Protein Sci. 1999 Sep;8(9):1765-72. PMID:10493577

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman
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