2z01: Difference between revisions
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==Crystal structure of phosphoribosylaminoimidazole synthetase from Geobacillus kaustophilus== | ==Crystal structure of phosphoribosylaminoimidazole synthetase from Geobacillus kaustophilus== | ||
<StructureSection load='2z01' size='340' side='right' caption='[[2z01]], [[Resolution|resolution]] 2.20Å' scene=''> | <StructureSection load='2z01' size='340' side='right'caption='[[2z01]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2z01]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2z01]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z01 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z01 OCA], [https://pdbe.org/2z01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z01 RCSB], [https://www.ebi.ac.uk/pdbsum/2z01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z01 ProSAT], [https://www.topsan.org/Proteins/RSGI/2z01 TOPSAN]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/PUR5_GEOKA PUR5_GEOKA] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z0/2z01_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z0/2z01_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z01 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 29: | Line 32: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Geobacillus kaustophilus]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Baba | [[Category: Baba S]] | ||
[[Category: Kanagawa | [[Category: Kanagawa M]] | ||
[[Category: Kawai | [[Category: Kawai G]] | ||
[[Category: Kuramitsu | [[Category: Kuramitsu S]] | ||
[[Category: Sampei G]] | |||
[[Category: Sampei | [[Category: Yokoyama S]] | ||
[[Category: Yokoyama | |||
Latest revision as of 12:13, 25 October 2023
Crystal structure of phosphoribosylaminoimidazole synthetase from Geobacillus kaustophilusCrystal structure of phosphoribosylaminoimidazole synthetase from Geobacillus kaustophilus
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedCrystal structures of 5-aminoimidazole ribonucleotide (AIR) synthetase, also known as PurM, from Thermus thermophilus (Tt) and Geobacillus kaustophilus (Gk) were determined. For TtPurM, the maximum resolution was 2.2 A and the space group was P21212 with four dimers in an asymmetric unit. For GkPurM, the maximum resolution was 2.2 A and the space group was P21212 with one monomer in asymmetric unit. The biological unit is dimer for both TtPurM and GkPurM and the dimer structures were similar to previously determined structures of PurM in general. For TtPurM, approximately 50 residues at the amino terminal were disordered in the crystal structure whereas, for GkPurM, the corresponding region covered the ATP-binding site forming an alpha helix in part, suggesting that the N-terminal region of PurM changes its conformation upon binding of ligands. FGAM binding site was predicted by the docking simulation followed by the MD simulation based on the SO4 2- binding site found in the crystal structure of TtPurM. Crystal structures and ligand binding of PurM proteins from Thermus thermophilus and Geobacillus kaustophilus.,Kanagawa M, Baba S, Watanabe Y, Nakagawa N, Ebihara A, Kuramitsu S, Yokoyama S, Sampei GI, Kawai G J Biochem. 2015 Oct 29. pii: mvv107. PMID:26515187[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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