2h5y: Difference between revisions

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[[Image:2h5y.jpg|left|200px]]
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{{STRUCTURE_2h5y|  PDB=2h5y  |  SCENE=  }}
'''Crystallographic structure of the Molybdate-Binding Protein of Xanthomonas citri at 1.7 Ang resolution bound to molybdate'''


==Crystallographic structure of the Molybdate-Binding Protein of Xanthomonas citri at 1.7 Ang resolution bound to molybdate==
<StructureSection load='2h5y' size='340' side='right'caption='[[2h5y]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2h5y]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri_pv._citri_str._306 Xanthomonas citri pv. citri str. 306]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H5Y FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h5y OCA], [https://pdbe.org/2h5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h5y RCSB], [https://www.ebi.ac.uk/pdbsum/2h5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h5y ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MODA_XANAC MODA_XANAC] Part of the ABC transporter complex ModABC involved in the transport of molybdenum into the cell (Probable). Binds molybdate and tungstate with high affinity in vitro (PubMed:16879982).<ref>PMID:16879982</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h5/2h5y_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h5y ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Xanthomonas axonopodis pv. citri ModA protein is the ABC periplasmic binding component responsible for the capture of molybdate. The protein was crystallized with sodium molybdate using the hanging-drop vapour-diffusion method in the presence of PEG or sulfate. X-ray diffraction data were collected to a maximum resolution of 1.7 A using synchrotron radiation. The crystal belongs to the orthorhombic space group C222(1), with unit-cell parameters a = 68.15, b = 172.14, c = 112.04 A. The crystal structure was solved by molecular-replacement methods and structure refinement is in progress.


==Overview==
Crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri.,Santacruz CP, Balan A, Ferreira LC, Barbosa JA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):289-91. Epub 2006 Feb 24. PMID:16511325<ref>PMID:16511325</ref>
Xanthomonas axonopodis pv. citri ModA protein is the ABC periplasmic binding component responsible for the capture of molybdate. The protein was crystallized with sodium molybdate using the hanging-drop vapour-diffusion method in the presence of PEG or sulfate. X-ray diffraction data were collected to a maximum resolution of 1.7 A using synchrotron radiation. The crystal belongs to the orthorhombic space group C222(1), with unit-cell parameters a = 68.15, b = 172.14, c = 112.04 A. The crystal structure was solved by molecular-replacement methods and structure refinement is in progress.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2H5Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_axonopodis_pv._citri Xanthomonas axonopodis pv. citri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5Y OCA].
</div>
<div class="pdbe-citations 2h5y" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri., Santacruz CP, Balan A, Ferreira LC, Barbosa JA, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):289-91. Epub 2006 Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511325 16511325]
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
[[Category: Single protein]]
== References ==
[[Category: Xanthomonas axonopodis pv. citri]]
<references/>
[[Category: Balan, A.]]
__TOC__
[[Category: Barbosa, J A.R G.]]
</StructureSection>
[[Category: Ferreira, L C.S.]]
[[Category: Large Structures]]
[[Category: Santacruz, C P.]]
[[Category: Xanthomonas citri pv. citri str. 306]]
[[Category: Moda]]
[[Category: Balan A]]
[[Category: Molybdate-binding protein]]
[[Category: Barbosa JARG]]
[[Category: Xanthomonas axonopodis pv. citri]]
[[Category: Ferreira LCS]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:54:13 2008''
[[Category: Santacruz CP]]

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