2h5y: Difference between revisions

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New page: left|200px<br /><applet load="2h5y" size="350" color="white" frame="true" align="right" spinBox="true" caption="2h5y, resolution 1.70Å" /> '''Crystallographic str...
 
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[[Image:2h5y.jpg|left|200px]]<br /><applet load="2h5y" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2h5y, resolution 1.70&Aring;" />
'''Crystallographic structure of the Molybdate-Binding Protein of Xanthomonas citri at 1.7 Ang resolution bound to molybdate'''<br />


==Overview==
==Crystallographic structure of the Molybdate-Binding Protein of Xanthomonas citri at 1.7 Ang resolution bound to molybdate==
Xanthomonas axonopodis pv. citri ModA protein is the ABC periplasmic, binding component responsible for the capture of molybdate. The protein, was crystallized with sodium molybdate using the hanging-drop, vapour-diffusion method in the presence of PEG or sulfate. X-ray, diffraction data were collected to a maximum resolution of 1.7 A using, synchrotron radiation. The crystal belongs to the orthorhombic space group, C222(1), with unit-cell parameters a = 68.15, b = 172.14, c = 112.04 A., The crystal structure was solved by molecular-replacement methods and, structure refinement is in progress.
<StructureSection load='2h5y' size='340' side='right'caption='[[2h5y]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2h5y]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri_pv._citri_str._306 Xanthomonas citri pv. citri str. 306]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H5Y FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h5y OCA], [https://pdbe.org/2h5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h5y RCSB], [https://www.ebi.ac.uk/pdbsum/2h5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h5y ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MODA_XANAC MODA_XANAC] Part of the ABC transporter complex ModABC involved in the transport of molybdenum into the cell (Probable). Binds molybdate and tungstate with high affinity in vitro (PubMed:16879982).<ref>PMID:16879982</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h5/2h5y_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h5y ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Xanthomonas axonopodis pv. citri ModA protein is the ABC periplasmic binding component responsible for the capture of molybdate. The protein was crystallized with sodium molybdate using the hanging-drop vapour-diffusion method in the presence of PEG or sulfate. X-ray diffraction data were collected to a maximum resolution of 1.7 A using synchrotron radiation. The crystal belongs to the orthorhombic space group C222(1), with unit-cell parameters a = 68.15, b = 172.14, c = 112.04 A. The crystal structure was solved by molecular-replacement methods and structure refinement is in progress.


==About this Structure==
Crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri.,Santacruz CP, Balan A, Ferreira LC, Barbosa JA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):289-91. Epub 2006 Feb 24. PMID:16511325<ref>PMID:16511325</ref>
2H5Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_axonopodis_pv._citri Xanthomonas axonopodis pv. citri] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=MOO:'>MOO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5Y OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri., Santacruz CP, Balan A, Ferreira LC, Barbosa JA, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):289-91. Epub 2006 Feb 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511325 16511325]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 2h5y" style="background-color:#fffaf0;"></div>
[[Category: Xanthomonas axonopodis pv. citri]]
[[Category: Balan, A.]]
[[Category: Barbosa, J.A.R.G.]]
[[Category: Ferreira, L.C.S.]]
[[Category: Santacruz, C.P.]]
[[Category: MOO]]
[[Category: SO4]]
[[Category: moda]]
[[Category: molybdate-binding protein]]
[[Category: xanthomonas axonopodis pv. citri]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:00:17 2008''
==See Also==
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Xanthomonas citri pv. citri str. 306]]
[[Category: Balan A]]
[[Category: Barbosa JARG]]
[[Category: Ferreira LCS]]
[[Category: Santacruz CP]]

Latest revision as of 11:50, 25 October 2023

Crystallographic structure of the Molybdate-Binding Protein of Xanthomonas citri at 1.7 Ang resolution bound to molybdateCrystallographic structure of the Molybdate-Binding Protein of Xanthomonas citri at 1.7 Ang resolution bound to molybdate

Structural highlights

2h5y is a 3 chain structure with sequence from Xanthomonas citri pv. citri str. 306. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MODA_XANAC Part of the ABC transporter complex ModABC involved in the transport of molybdenum into the cell (Probable). Binds molybdate and tungstate with high affinity in vitro (PubMed:16879982).[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Xanthomonas axonopodis pv. citri ModA protein is the ABC periplasmic binding component responsible for the capture of molybdate. The protein was crystallized with sodium molybdate using the hanging-drop vapour-diffusion method in the presence of PEG or sulfate. X-ray diffraction data were collected to a maximum resolution of 1.7 A using synchrotron radiation. The crystal belongs to the orthorhombic space group C222(1), with unit-cell parameters a = 68.15, b = 172.14, c = 112.04 A. The crystal structure was solved by molecular-replacement methods and structure refinement is in progress.

Crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri.,Santacruz CP, Balan A, Ferreira LC, Barbosa JA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):289-91. Epub 2006 Feb 24. PMID:16511325[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Balan A, Santacruz CP, Moutran A, Ferreira RC, Medrano FJ, Pérez CA, Ramos CH, Ferreira LC. The molybdate-binding protein (ModA) of the plant pathogen Xanthomonas axonopodis pv. citri. Protein Expr Purif. 2006 Dec;50(2):215-22. PMID:16879982 doi:10.1016/j.pep.2006.06.014
  2. ↑ Santacruz CP, Balan A, Ferreira LC, Barbosa JA. Crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):289-91. Epub 2006 Feb 24. PMID:16511325 doi:10.1107/S1744309106003812

2h5y, resolution 1.70Å

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