2e40: Difference between revisions

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-[[Image:2e40.gif|left|200px]]<br /><applet load="2e40" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2e40, resolution 1.90&Aring;" />
-'''Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium in complex with gluconolactone'''<br />
-==Overview==
+==Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium in complex with gluconolactone==
+<StructureSection load='2e40' size='340' side='right'caption='[[2e40]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2e40]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E40 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LGC:(3S,4R,5R,6S)-3,4,5-TRIHYDROXY-6-(HYDROXYMETHYL)TETRAHYDRO-2H-PYRAN-2-ONE'>LGC</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e40 OCA], [https://pdbe.org/2e40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e40 RCSB], [https://www.ebi.ac.uk/pdbsum/2e40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e40 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/BGL1A_PHACH BGL1A_PHACH] Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.<ref>PMID:16896601</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e4/2e40_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e40 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
 The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconolactone complexed forms. The overall structure and the characteristic of subsite -1 (glycone site) were similar to those of other known GH1 enzymes. The loop regions covering on the (beta/alpha)(8) barrel was significantly deviated, and they form a unique subsite +1 (aglycone site) of BGL1A.
-==About this Structure==
+Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium.,Nijikken Y, Tsukada T, Igarashi K, Samejima M, Wakagi T, Shoun H, Fushinobu S FEBS Lett. 2007 Apr 3;581(7):1514-20. Epub 2007 Mar 13. PMID:17376440<ref>PMID:17376440</ref>
-E40 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with <scene name='pdbligand=LGC:'>LGC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E40 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium., Nijikken Y, Tsukada T, Igarashi K, Samejima M, Wakagi T, Shoun H, Fushinobu S, FEBS Lett. 2007 Apr 3;581(7):1514-20. Epub 2007 Mar 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17376440 17376440]
+</div>
-[[Category: Beta-glucosidase]]
+<div class="pdbe-citations 2e40" style="background-color:#fffaf0;"></div>
-[[Category: Phanerochaete chrysosporium]]
-[[Category: Single protein]]
-[[Category: Fushinobu, S.]]
-[[Category: Igarashi, K.]]
-[[Category: Nijikken, Y.]]
-[[Category: Samejima, M.]]
-[[Category: Tsukada, T.]]
-[[Category: LGC]]
-[[Category: clan gh-a]]
-[[Category: glycoside hydrolase family 1]]
-[[Category: national project on protein structural and functional analyses]]
-[[Category: nppsfa]]
-[[Category: structural genomics]]
-[[Category: tim barrel]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:05:46 2008''
+==See Also==
+*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Phanerodontia chrysosporium]]
+[[Category: Fushinobu S]]
+[[Category: Igarashi K]]
+[[Category: Nijikken Y]]
+[[Category: Samejima M]]
+[[Category: Tsukada T]]