2e1m: Difference between revisions
New page: left|200px<br /><applet load="2e1m" size="350" color="white" frame="true" align="right" spinBox="true" caption="2e1m, resolution 2.80Å" /> '''Crystal Structure of... |
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== | ==Crystal Structure of L-Glutamate Oxidase from Streptomyces sp. X-119-6== | ||
<StructureSection load='2e1m' size='340' side='right'caption='[[2e1m]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
[ | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2e1m]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._X-119-6 Streptomyces sp. X-119-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E1M FirstGlance]. <br> | |||
[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e1m OCA], [https://pdbe.org/2e1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e1m RCSB], [https://www.ebi.ac.uk/pdbsum/2e1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e1m ProSAT]</span></td></tr> | ||
[[ | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8L3C7_9ACTN Q8L3C7_9ACTN] | |||
[ | == Evolutionary Conservation == | ||
[[ | [[Image:Consurf_key_small.gif|200px|right]] | ||
[ | Check<jmol> | ||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/2e1m_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e1m ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
L-Glutamate oxidase (LGOX) from Streptomyces sp. X-119-6, which catalyzes the oxidative deamination of L-glutamate, has attracted increasing attention as a component of amperometric L-glutamate sensors used in the food industry and clinical biochemistry. The precursor of LGOX, which has a homodimeric structure, is less active than the mature enzyme with an alpha(2)beta(2)V(2) structure; enzymatic proteolysis of the precursor forms the stable mature enzyme. We solved the crystal structure of mature LGOX using molecular replacement with a structurally homologous model of L-amino acid oxidase (LAAO) from snake venom: LGOX has a deeply buried active site and two entrances from the surface of the protein into the active site. Comparison of the LGOX structure with that of LAAO revealed that LGOX has three regions that are absent from the LAAO structure, one of which is involved in the formation of the entrance. Furthermore, the arrangement of the residues composing the active site differs between LGOX and LAAO, and the active site of LGOX is narrower than that of LAAO. Results of the comparative analyses described herein raise the possibility that such a unique structure of LGOX is associated with its substrate specificity. | |||
Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6.,Arima J, Sasaki C, Sakaguchi C, Mizuno H, Tamura T, Kashima A, Kusakabe H, Sugio S, Inagaki K FEBS J. 2009 Jul;276(14):3894-903. Epub 2009 Jun 15. PMID:19531050<ref>PMID:19531050</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2e1m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptomyces sp. X-119-6]] | |||
[[Category: Arima J]] | |||
[[Category: Inagaki K]] | |||
[[Category: Kashima A]] | |||
[[Category: Kusakabe H]] | |||
[[Category: Mizuno H]] | |||
[[Category: Sakaguchi C]] | |||
[[Category: Sasaki C]] | |||
[[Category: Sugio S]] | |||
[[Category: Tamura T]] | |||
