1xb7: Difference between revisions

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-[[Image:1xb7.png|left|200px]]
-{{STRUCTURE_1xb7|  PDB=1xb7  |  SCENE=  }}
+==X-ray structure of ERRalpha LBD in complex with a PGC-1alpha peptide at 2.5A resolution==
+<StructureSection load='1xb7' size='340' side='right'caption='[[1xb7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1xb7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XB7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xb7 OCA], [https://pdbe.org/1xb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xb7 RCSB], [https://www.ebi.ac.uk/pdbsum/1xb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xb7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ERR1_HUMAN ERR1_HUMAN] Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.<ref>PMID:9271417</ref> <ref>PMID:12522104</ref> <ref>PMID:16150865</ref> <ref>PMID:17676930</ref> <ref>PMID:18063693</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xb/1xb7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xb7 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structure of the ligand binding domain (LBD) of the estrogen-related receptor alpha (ERRalpha, NR3B1) complexed with a coactivator peptide from peroxisome proliferator-activated receptor coactivator-1alpha (PGC-1alpha) reveals a transcriptionally active conformation in the absence of a ligand. This is the first x-ray structure of ERRalpha LBD, solved to a resolution of 2.5 A, and the first structure of a PGC-1alpha complex. The putative ligand binding pocket (LBP) of ERRalpha is almost completely occupied by side chains, in particular with the bulky side chain of Phe328 (corresponding to Ala272 in ERRgamma and Ala350 in estrogen receptor alpha). Therefore, a ligand of a size equivalent to more than approximately 4 carbon atoms could only bind in the LBP, if ERRalpha would undergo a major conformational change (in particular the ligand would displace H12 from its agonist position). The x-ray structure thus provides strong evidence for ligand-independent transcriptional activation by ERRalpha. The interactions of PGC-1alpha with ERRalpha also reveal for the first time the atomic details of how a coactivator peptide containing an inverted LXXLL motif (namely a LLXYL motif) binds to a LBD. In addition, we show that a PGC-1alpha peptide containing this nuclear box motif from the L3 site binds ERRalpha LBD with a higher affinity than a peptide containing a steroid receptor coactivator-1 motif and that the affinity is further enhanced when all three leucine-rich regions of PGC-1alpha are present.
-===X-ray structure of ERRalpha LBD in complex with a PGC-1alpha peptide at 2.5A resolution===
+Evidence for ligand-independent transcriptional activation of the human estrogen-related receptor alpha (ERRalpha): crystal structure of ERRalpha ligand binding domain in complex with peroxisome proliferator-activated receptor coactivator-1alpha.,Kallen J, Schlaeppi JM, Bitsch F, Filipuzzi I, Schilb A, Riou V, Graham A, Strauss A, Geiser M, Fournier B J Biol Chem. 2004 Nov 19;279(47):49330-7. Epub 2004 Aug 26. PMID:15337744<ref>PMID:15337744</ref>
-{{ABSTRACT_PUBMED_15337744}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1xb7" style="background-color:#fffaf0;"></div>
-[[1xb7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XB7 OCA].
 ==See Also==
-*[[Estrogen-related receptor|Estrogen-related receptor]]
+*[[Estrogen-related receptor 3D structures|Estrogen-related receptor 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:015337744</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Bitsch, F.]]
+[[Category: Large Structures]]
-[[Category: Filipuzzi, I.]]
+[[Category: Bitsch F]]
-[[Category: Fournier, B.]]
+[[Category: Filipuzzi I]]
-[[Category: Geiser, M.]]
+[[Category: Fournier B]]
-[[Category: Graham, A.]]
+[[Category: Geiser M]]
-[[Category: Kallen, J.]]
+[[Category: Graham A]]
-[[Category: Riou, V.]]
+[[Category: Kallen J]]
-[[Category: Schilb, A.]]
+[[Category: Riou V]]
-[[Category: Schlaeppi, J M.]]
+[[Category: Schilb A]]
-[[Category: Strauss, A.]]
+[[Category: Schlaeppi JM]]
-[[Category: Amphipathic alpha-helix]]
+[[Category: Strauss A]]
-[[Category: Coactivator peptide]]
-[[Category: Ligand binding domain]]
-[[Category: Nuclear hormone receptor]]
-[[Category: Three-layered alpha-helical sandwich]]
-[[Category: Transcription]]