1wl0: Difference between revisions

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New page: left|200px<br /><applet load="1wl0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wl0, resolution 3.2Å" /> '''Crystal Structure Of ...
 
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[[Image:1wl0.gif|left|200px]]<br /><applet load="1wl0" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1wl0, resolution 3.2&Aring;" />
'''Crystal Structure Of Octaprenyl Pyrophosphate Synthase From Hyperthermophilic Thermotoga Maritima R44A mutant'''<br />


==Overview==
==Crystal Structure Of Octaprenyl Pyrophosphate Synthase From Hyperthermophilic Thermotoga Maritima R44A mutant==
Octaprenyl pyrophosphate synthase (OPPs) catalyzes the condensation of, five isopentenyl pyrophosphates with farnesyl pyrophosphate to generate, C(40) octaprenyl pyrophosphate. The enzymes from the hyperthermophilic, bacterium Thermotoga maritima and from the mesophilic Escherichia coli, were expressed in E. coli and the recombinant proteins were purified and, crystallized. The T. maritima OPPs crystals belong to space group P42(1)2, with unit-cell parameters a = b = 151.53, c = 69.72 A. The E. coli OPPs, crystals belong to space group C222(1), with unit-cell parameters a =, 247.66, b = 266.10, c = 157.93 A. Diffraction data were collected at 100 K, using synchrotron radiation and an in-house X-ray source. Structure, determination of T. maritima OPPs has been carried out using MIR data sets, at 2.8 A resolution. The asymmetric unit contains one dimer. An initial, model with 280 residues per subunit has been built and refined to 2.28 A, resolution. It shows mostly helical structure and resembles that of avian, farnesyl pyrophosphate synthase.
<StructureSection load='1wl0' size='340' side='right'caption='[[1wl0]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1wl0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WL0 FirstGlance]. <br>
1WL0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima_msb8 Thermotoga maritima msb8] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Trans-octaprenyltranstransferase Trans-octaprenyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.11 2.5.1.11] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WL0 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wl0 OCA], [https://pdbe.org/1wl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wl0 RCSB], [https://www.ebi.ac.uk/pdbsum/1wl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wl0 ProSAT]</span></td></tr>
Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli., Guo RT, Ko TP, Chou CC, Shr HL, Chu HM, Tsai YH, Liang PH, Wang AH, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2265-8. Epub 2003, Nov 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14646090 14646090]
</table>
[[Category: Single protein]]
== Function ==
[[Category: Thermotoga maritima msb8]]
[https://www.uniprot.org/uniprot/Q9X1M1_THEMA Q9X1M1_THEMA]  
[[Category: Trans-octaprenyltranstransferase]]
== Evolutionary Conservation ==
[[Category: Cheng, Y.L.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Cheng, Y.S.]]
Check<jmol>
[[Category: Guo, R.T.]]
  <jmolCheckbox>
[[Category: Kuo, C.J.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wl/1wl0_consurf.spt"</scriptWhenChecked>
[[Category: Liang, P.H.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Wang, A.H.J.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: SO4]]
  </jmolCheckbox>
[[Category: thermophilic]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wl0 ConSurf].
[[Category: trans-type prenyltransferase]]
<div style="clear:both"></div>
 
__TOC__
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:08:38 2007''
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermotoga maritima MSB8]]
[[Category: Cheng YL]]
[[Category: Cheng YS]]
[[Category: Guo RT]]
[[Category: Kuo CJ]]
[[Category: Liang PH]]
[[Category: Wang AH-J]]

Latest revision as of 10:56, 25 October 2023

Crystal Structure Of Octaprenyl Pyrophosphate Synthase From Hyperthermophilic Thermotoga Maritima R44A mutantCrystal Structure Of Octaprenyl Pyrophosphate Synthase From Hyperthermophilic Thermotoga Maritima R44A mutant

Structural highlights

1wl0 is a 2 chain structure with sequence from Thermotoga maritima MSB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9X1M1_THEMA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1wl0, resolution 3.20Å

Drag the structure with the mouse to rotate

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OCA
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