1tzt: Difference between revisions

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-{{Seed}}
-[[Image:1tzt.png|left|200px]]
-<!--
+==T. maritima NusB, P21==
-The line below this paragraph, containing "STRUCTURE_1tzt", creates the "Structure Box" on the page.
+<StructureSection load='1tzt' size='340' side='right'caption='[[1tzt]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1tzt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TZT FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_1tzt|  PDB=1tzt  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tzt OCA], [https://pdbe.org/1tzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tzt RCSB], [https://www.ebi.ac.uk/pdbsum/1tzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tzt ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NUSB_THEMA NUSB_THEMA] Involved in the transcription termination process (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tz/1tzt_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tzt ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+NusB is a prokaryotic transcription factor involved in antitermination processes, during which it interacts with the boxA portion of the mRNA nut site. Previous studies have shown that NusB exhibits an all-helical fold, and that the protein from Escherichia coli forms monomers, while Mycobacterium tuberculosis NusB is a dimer. The functional significance of NusB dimerization is unknown. We have determined five crystal structures of NusB from Thermotoga maritima. In three crystal forms the protein appeared monomeric, whereas the two other crystal forms contained assemblies, which resembled the M. tuberculosis dimers. In solution, T. maritima NusB could be cross-linked as dimers, but it migrated as a monomer in gel-filtration analyses, suggesting a monomer/dimer equilibrium with a preference for the monomer. Binding to boxA-like RNA sequences could be detected by gel-shift analyses and UV-induced cross-linking. An N-terminal arginine-rich sequence is a probable RNA binding site of the protein, exhibiting aromatic residues as potential stacking partners for the RNA bases. Anions located in various structures support the assignment of this RNA binding site. The proposed RNA binding region is hidden in the subunit interface of dimeric NusB proteins, such as NusB from M. tuberculosis, suggesting that such dimers have to undergo a considerable conformational change or dissociate for engagement with RNA. Therefore, in certain organisms, dimerization may be employed to package NusB in an inactive form until recruitment into antitermination complexes.
-===T. maritima NusB, P21===
+Crystal structures of the antitermination factor NusB from Thermotoga maritima and implications for RNA binding.,Bonin I, Robelek R, Benecke H, Urlaub H, Bacher A, Richter G, Wahl MC Biochem J. 2004 Nov 1;383(Pt. 3):419-28. PMID:15279620<ref>PMID:15279620</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_15279620}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1tzt" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 15279620 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_15279620}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-TZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZT OCA].
-==Reference==
-Crystal structures of the antitermination factor NusB from Thermotoga maritima and implications for RNA binding., Bonin I, Robelek R, Benecke H, Urlaub H, Bacher A, Richter G, Wahl MC, Biochem J. 2004 Nov 1;383(Pt. 3):419-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15279620 15279620]
-[[Category: Single protein]]
 [[Category: Thermotoga maritima]]
-[[Category: Bacher, A.]]
+[[Category: Bacher A]]
-[[Category: Benecke, H.]]
+[[Category: Benecke H]]
-[[Category: Bonin, I.]]
+[[Category: Bonin I]]
-[[Category: Richter, G.]]
+[[Category: Richter G]]
-[[Category: Robelek, R.]]
+[[Category: Robelek R]]
-[[Category: Urlaub, H.]]
+[[Category: Urlaub H]]
-[[Category: Wahl, M C.]]
+[[Category: Wahl MC]]
-[[Category: N-utilization substance]]
-[[Category: Nusb]]
-[[Category: Rna-protein interaction]]
-[[Category: Transcription regulation]]
-[[Category: Transcriptional antitermination]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:39:27 2008''