8q5a: Difference between revisions

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-'''Unreleased structure'''
-The entry 8q5a is ON HOLD
+==Crystal structure of metal-dependent class II sulfofructosephosphate aldolase from Hafnia paralvei HpSqiA-Zn in complex with dihydroxyacetone phosphate (DHAP)==
+<StructureSection load='8q5a' size='340' side='right'caption='[[8q5a]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8q5a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hafnia_paralvei Hafnia paralvei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Q5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Q5A FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=13P:1,3-DIHYDROXYACETONEPHOSPHATE'>13P</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8q5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8q5a OCA], [https://pdbe.org/8q5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8q5a RCSB], [https://www.ebi.ac.uk/pdbsum/8q5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8q5a ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A2A2MA06_9GAMM A0A2A2MA06_9GAMM]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Sulfoquinovose (SQ, 6-deoxy-6-sulfoglucose) is a sulfosugar that is the anionic head group of plant, algal and cyanobacterial sulfolipids: sulfoquinovosyl diacylglycerols. SQ is produced within photosynthetic tissues, forms a major terrestrial reservoir of biosulfur, and is an important species within the biogeochemical sulfur cycle. A major pathway for SQ breakdown is the sulfoglycolytic Embden-Meyerhof-Parnas (sulfo-EMP) pathway, which involves cleavage of the 6-carbon chain of the intermediate sulfofructose-1-phosphate (SFP) into dihydroxyacetone and sulfolactaldehyde, catalyzed by class I or II SFP aldolases. While the molecular basis of catalysis is understood for class I SFP aldolases, comparatively little is known about class II SFP aldolases. Here, we report the molecular architecture and biochemical basis of catalysis of two metal-dependent class II SFP aldolases from Hafnia paralvei and Yersinia aldovae. 3D X-ray structures of complexes with substrate SFP and product DHAP reveal a dimer-of-dimers (tetrameric) assembly, the sulfonate binding pocket, two metal binding sites, and flexible loops that are implicated in catalysis. Both enzymes were metal dependent and exhibited high K(M) values for SFP, consistent with their role in a unidirectional nutrient acquisition pathway. Bioinformatic analysis identified a range of sulfo-EMP gene clusters containing class I/II SFP aldolases. The class I and II SFP aldolases occur exclusively within Actinobacteria and Firmicutes phyla, respectively, while both classes of enzyme occur within Proteobacteria. This work emphasizes the importance of SQ as a nutrient for diverse bacterial phyla and the different chemical strategies they use to harvest carbon from this sulfosugar.
-Authors: Sharma, M., Davies, G.J.
+Defining the molecular architecture, metal dependence, and distribution of metal-dependent class II sulfofructose-1-phosphate aldolases.,Sharma M, Kaur A, Soler NM, Lingford JP, Epa R, Goddard-Borger ED, Davies GJ, Williams SJ J Biol Chem. 2023 Oct 12:105338. doi: 10.1016/j.jbc.2023.105338. PMID:37838169<ref>PMID:37838169</ref>
-Description: Crystal structure of metal-dependent class II sulfofructosephosphate aldolase from Hafnia paralvei HpSqiA-Zn in complex with dihydroxyacetone phosphate (DHAP)
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Sharma, M]]
+<div class="pdbe-citations 8q5a" style="background-color:#fffaf0;"></div>
-[[Category: Davies, G.J]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Hafnia paralvei]]
+[[Category: Large Structures]]
+[[Category: Davies GJ]]
+[[Category: Sharma M]]