5lio: Difference between revisions
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The | ==Lysozyme, collected at rotation 360 degree per second== | ||
<StructureSection load='5lio' size='340' side='right'caption='[[5lio]], [[Resolution|resolution]] 1.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5lio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LIO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.496Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lio OCA], [https://pdbe.org/5lio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lio RCSB], [https://www.ebi.ac.uk/pdbsum/5lio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lio ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 x 75 microm, frame rates of up to 3000 Hz and a dead time as low as 3.8 micros. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine varphi-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed. | |||
EIGER detector: application in macromolecular crystallography.,Casanas A, Warshamanage R, Finke AD, Panepucci E, Olieric V, Noll A, Tampe R, Brandstetter S, Forster A, Mueller M, Schulze-Briese C, Bunk O, Wang M Acta Crystallogr D Struct Biol. 2016 Sep;72(Pt 9):1036-48. doi:, 10.1107/S2059798316012304. Epub 2016 Aug 31. PMID:27599736<ref>PMID:27599736</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5lio" style="background-color:#fffaf0;"></div> | ||
[[Category: Finke | |||
[[Category: | ==See Also== | ||
*[[Lysozyme 3D structures|Lysozyme 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Gallus gallus]] | |||
[[Category: Large Structures]] | |||
[[Category: Casanas A]] | |||
[[Category: Finke A]] | |||
[[Category: Wang M]] | |||