2y7h: Difference between revisions

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[[Image:2y7h.jpg|left|200px]]


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==Atomic model of the DNA-bound methylase complex from the Type I restriction-modification enzyme EcoKI (M2S1). Based on fitting into EM map 1534.==
The line below this paragraph, containing "STRUCTURE_2y7h", creates the "Structure Box" on the page.
<SX load='2y7h' size='340' side='right' viewer='molstar' caption='[[2y7h]], [[Resolution|resolution]] 18.00&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2y7h]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y7H FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 18&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
{{STRUCTURE_2y7h|  PDB=2y7h  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y7h OCA], [https://pdbe.org/2y7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y7h RCSB], [https://www.ebi.ac.uk/pdbsum/2y7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y7h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T1SK_ECOLI T1SK_ECOLI]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type-I DNA restriction-modification (R/M) systems are important agents in limiting the transmission of mobile genetic elements responsible for spreading bacterial resistance to antibiotics. EcoKI, a Type I R/M enzyme from Escherichia coli, acts by methylation- and sequence-specific recognition, leading to either methylation of DNA or translocation and cutting at a random site, often hundreds of base pairs away. Consisting of one specificity subunit, two modification subunits, and two DNA translocase/endonuclease subunits, EcoKI is inhibited by the T7 phage antirestriction protein ocr, a DNA mimic. We present a 3D density map generated by negative-stain electron microscopy and single particle analysis of the central core of the restriction complex, the M.EcoKI M(2)S(1) methyltransferase, bound to ocr. We also present complete atomic models of M.EcoKI in complex with ocr and its cognate DNA giving a clear picture of the overall clamp-like operation of the enzyme. The model is consistent with a large body of experimental data on EcoKI published over 40 years.


===ATOMIC MODEL OF THE DNA-BOUND METHYLASE COMPLEX FROM THE TYPE I RESTRICTION-MODIFICATION ENZYME ECOKI (M2S1). BASED ON FITTING INTO EM MAP 1534.===
The structure of M.EcoKI Type I DNA methyltransferase with a DNA mimic antirestriction protein.,Kennaway CK, Obarska-Kosinska A, White JH, Tuszynska I, Cooper LP, Bujnicki JM, Trinick J, Dryden DT Nucleic Acids Res. 2009 Feb;37(3):762-70. Epub 2008 Dec 11. PMID:19074193<ref>PMID:19074193</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 19074193 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_19074193}}
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</SX>
==About this Structure==
[[2y7h]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y7H OCA].
 
==Reference==
<ref group="xtra">PMID:19074193</ref><references group="xtra"/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Type I site-specific deoxyribonuclease]]
[[Category: Large Structures]]
[[Category: Bujnicki, J M.]]
[[Category: Bujnicki JM]]
[[Category: Cooper, L P.]]
[[Category: Cooper LP]]
[[Category: Dryden, D T.F.]]
[[Category: Dryden DTF]]
[[Category: Kennaway, C K.]]
[[Category: Kennaway CK]]
[[Category: Obarska-Kosinska, A.]]
[[Category: Obarska-Kosinska A]]
[[Category: Trinick, J.]]
[[Category: Trinick J]]
[[Category: Tuszynska, I.]]
[[Category: Tuszynska I]]
[[Category: White, J H.]]
[[Category: White JH]]

Latest revision as of 21:17, 18 October 2023

Atomic model of the DNA-bound methylase complex from the Type I restriction-modification enzyme EcoKI (M2S1). Based on fitting into EM map 1534.Atomic model of the DNA-bound methylase complex from the Type I restriction-modification enzyme EcoKI (M2S1). Based on fitting into EM map 1534.

2y7h, resolution 18.00Å

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