7tzk: Difference between revisions
New page: '''Unreleased structure''' The entry 7tzk is ON HOLD Authors: Description: Category: Unreleased Structures |
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==EPS8 SH3 Domain with NleH1 PxxDY Motif== | |||
<StructureSection load='7tzk' size='340' side='right'caption='[[7tzk]], [[Resolution|resolution]] 1.43Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7tzk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O127:H6_str._E2348/69 Escherichia coli O127:H6 str. E2348/69] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TZK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.43Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tzk OCA], [https://pdbe.org/7tzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tzk RCSB], [https://www.ebi.ac.uk/pdbsum/7tzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tzk ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/EPS8_HUMAN EPS8_HUMAN] Upon binding to EGF receptor/EGFR enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Attaching and effacing (AE) lesion formation on enterocytes by enteropathogenic Escherichia coli (EPEC) requires the EPEC type III secretion system (T3SS). Two T3SS effectors injected into the host cell during infection are the atypical kinases, NleH1 and NleH2. However, the host targets of NleH1 and NleH2 kinase activity during infection have not been reported. Here phosphoproteomics identified Ser775 in the microvillus protein Eps8 as a bona fide target of NleH1 and NleH2 phosphorylation. Both kinases interacted with Eps8 through previously unrecognized, noncanonical "proline-rich" motifs, PxxDY, that bound the Src Homology 3 (SH3) domain of Eps8. Structural analysis of the Eps8 SH3 domain bound to a peptide containing one of the proline-rich motifs from NleH showed that the N-terminal part of the peptide adopts a type II polyproline helix, and its C-terminal "DY" segment makes multiple contacts with the SH3 domain. Ser775 phosphorylation by NleH1 or NleH2 hindered Eps8 bundling activity and drove dispersal of Eps8 from the AE lesion during EPEC infection. This finding suggested that NleH1 and NleH2 altered the cellular localization of Eps8 and the cytoskeletal composition of AE lesions during EPEC infection. | |||
Targeting of microvillus protein Eps8 by the NleH effector kinases from enteropathogenic E. coli.,Pollock GL, Grishin AM, Giogha C, Gan J, Oates CV, McMillan PJ, Gaeta I, Tyska MJ, Pearson JS, Scott NE, Cygler M, Hartland EL Proc Natl Acad Sci U S A. 2022 Aug 23;119(34):e2204332119. doi:, 10.1073/pnas.2204332119. Epub 2022 Aug 17. PMID:35976880<ref>PMID:35976880</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7tzk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli O127:H6 str. E2348/69]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Cygler M]] | |||
[[Category: Grishin AM]] | |||