7rfv: Difference between revisions
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==Tailspike protein 4 (TSP4) from phage CBA120, residues 1-250, obtained in the presence of PEG8000== | |||
<StructureSection load='7rfv' size='340' side='right'caption='[[7rfv]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7rfv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_CBA120 Escherichia virus CBA120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RFV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rfv OCA], [https://pdbe.org/7rfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rfv RCSB], [https://www.ebi.ac.uk/pdbsum/7rfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rfv ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G3M192_9CAUD G3M192_9CAUD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Four tailspike proteins (TSP1-4) of Escherichia coli O157:H7 bacteriophage CBA120 enable infection of multiple hosts. They form a branched complex that attaches to the tail baseplate. Each TSP recognizes a different lipopolysaccharide on the membrane of a different bacterial host. The 335 N-terminal residues of TSP4 promote the assembly of the TSP complex and anchor it to the tail baseplate. The crystal structure of TSP4-N335 reveals a trimeric protein comprising four domains. The baseplate anchor domain (AD) contains an intertwined triple-stranded beta-helix. The ensuing XD1, XD2 and XD3 beta-sheet containing domains mediate the binding of TSP1-3 to TSP4. Each of the XD domains adopts the same fold as the respective XD domains of bacteriophage T4 gp10 baseplate protein, known to engage in protein-protein interactions via its XD2 and XD3 domains. The structural similarity suggests that XD2 and XD3 of TSP4 also function in protein-protein interactions. Analytical ultracentrifugation analyses of TSP4-N335 and of domain deletion proteins showed how TSP4-N335 promotes the formation of the TSP quaternary complex. TSP1 and TSP2 bind directly to TSP4 whereas TSP3 binding requires a pre-formed TSP4-N335:TSP2 complex. A 3-dimensional model of the bacteriophage CBA120 TSP complex has been developed based on the structural and ultracentrifuge information. | |||
Structure of Escherichia coli O157:H7 bacteriophage CBA120 tailspike protein 4 baseplate anchor and tailspike assembly domains (TSP4-N).,Chao KL, Shang X, Greenfield J, Linden SB, Alreja AB, Nelson DC, Herzberg O Sci Rep. 2022 Feb 8;12(1):2061. doi: 10.1038/s41598-022-06073-2. PMID:35136138<ref>PMID:35136138</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7rfv" style="background-color:#fffaf0;"></div> | ||
[[Category: Grenfield | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Tailspike protein|Tailspike protein]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia virus CBA120]] | |||
[[Category: Large Structures]] | |||
[[Category: Chao K]] | |||
[[Category: Grenfield J]] | |||
[[Category: Herzberg O]] | |||
[[Category: Linden SB]] | |||
[[Category: Nelson DC]] | |||
[[Category: Shang X]] | |||