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==crystal structure of ligand-free PLEKHA7 PH domain== | ==crystal structure of ligand-free PLEKHA7 PH domain== | ||
<StructureSection load='7kk7' size='340' side='right'caption='[[7kk7]]' scene=''> | <StructureSection load='7kk7' size='340' side='right'caption='[[7kk7]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KK7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7kk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KK7 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kk7 OCA], [https://pdbe.org/7kk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kk7 RCSB], [https://www.ebi.ac.uk/pdbsum/7kk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kk7 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kk7 OCA], [https://pdbe.org/7kk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kk7 RCSB], [https://www.ebi.ac.uk/pdbsum/7kk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kk7 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/PKHA7_HUMAN PKHA7_HUMAN] Required for zonula adherens biogenesis and maintenance (PubMed:19041755). Acts via its interaction with CAMSAP3, which anchors microtubules at their minus-ends to zonula adherens, leading to the recruitment of KIFC3 kinesin to the junctional site (PubMed:19041755). Mediates docking of ADAM10 to zonula adherens through a PDZD11-dependent interaction with the ADAM10-binding protein TSPAN33 (PubMed:30463011).<ref>PMID:19041755</ref> <ref>PMID:30463011</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
PLEKHA7 (pleckstrin homology domain containing family A member 7) plays key roles in intracellular signaling, cytoskeletal organization, and cell adhesion, and is associated with multiple human cancers. The interactions of its pleckstrin homology (PH) domain with membrane phosphatidyl-inositol-phosphate (PIP) lipids are critical for proper cellular localization and function, but little is known about how PLEKHA7 and other PH domains interact with membrane-embedded PIPs. Here we describe the structural basis for recognition of membrane-bound PIPs by PLEHA7. Using X-ray crystallography, nuclear magnetic resonance, molecular dynamics simulations, and isothermal titration calorimetry, we show that the interaction of PLEKHA7 with PIPs is multivalent, distinct from a discrete one-to-one interaction, and induces PIP clustering. Our findings reveal a central role of the membrane assembly in mediating protein-PIP association and provide a roadmap for understanding how the PH domain contributes to the signaling, adhesion, and nanoclustering functions of PLEKHA7. | |||
Structural basis for the association of PLEKHA7 with membrane-embedded phosphatidylinositol lipids.,Aleshin AE, Yao Y, Iftikhar A, Bobkov AA, Yu J, Cadwell G, Klein MG, Dong C, Bankston LA, Liddington RC, Im W, Powis G, Marassi FM Structure. 2021 Apr 13. pii: S0969-2126(21)00116-7. doi:, 10.1016/j.str.2021.03.018. PMID:33878292<ref>PMID:33878292</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7kk7" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Aleshin AE]] | [[Category: Aleshin AE]] | ||
[[Category: Liddington RC]] | [[Category: Liddington RC]] | ||
[[Category: Marassi FM]] | [[Category: Marassi FM]] | ||