7jie: Difference between revisions
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==Structure of GII.4 P-domain in Complex with NORO-320 FAB== | |||
<StructureSection load='7jie' size='340' side='right'caption='[[7jie]], [[Resolution|resolution]] 2.25Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7jie]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Norovirus_Hu/GII.4/Sydney/NSW0514/2012/AU Norovirus Hu/GII.4/Sydney/NSW0514/2012/AU]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JIE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.254Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jie OCA], [https://pdbe.org/7jie PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jie RCSB], [https://www.ebi.ac.uk/pdbsum/7jie PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jie ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/K4LM89_9CALI K4LM89_9CALI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The rational development of norovirus vaccine candidates requires a deep understanding of the antigenic diversity and mechanisms of neutralization of the virus. Here, we isolate and characterize a panel of broadly cross-reactive naturally occurring human monoclonal IgMs, IgAs and IgGs reactive with human norovirus (HuNoV) genogroup I or II (GI or GII). We note three binding patterns and identify monoclonal antibodies (mAbs) that neutralize at least one GI or GII HuNoV strain when using a histo-blood group antigen (HBGA) blocking assay. The HBGA blocking assay and a virus neutralization assay using human intestinal enteroids reveal that the GII-specific mAb NORO-320, mediates HBGA blocking and neutralization of multiple GII genotypes. The Fab form of NORO-320 neutralizes GII.4 infection more potently than the mAb, however, does not block HBGA binding. The crystal structure of NORO-320 Fab in complex with GII.4 P-domain shows that the antibody recognizes a highly conserved region in the P-domain distant from the HBGA binding site. Dynamic light scattering analysis of GII.4 virus-like particles with mAb NORO-320 shows severe aggregation, suggesting neutralization is by steric hindrance caused by multivalent cross-linking. Aggregation was not observed with the Fab form of NORO-320, suggesting that this clone also has additional inhibitory features. | |||
Broadly cross-reactive human antibodies that inhibit genogroup I and II noroviruses.,Alvarado G, Salmen W, Ettayebi K, Hu L, Sankaran B, Estes MK, Venkataram Prasad BV, Crowe JE Jr Nat Commun. 2021 Jul 14;12(1):4320. doi: 10.1038/s41467-021-24649-w. PMID:34262046<ref>PMID:34262046</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Hu | <div class="pdbe-citations 7jie" style="background-color:#fffaf0;"></div> | ||
[[Category: Prasad | |||
[[Category: Salmen | ==See Also== | ||
*[[Antibody 3D structures|Antibody 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Norovirus Hu/GII 4/Sydney/NSW0514/2012/AU]] | |||
[[Category: Hu L]] | |||
[[Category: Prasad B]] | |||
[[Category: Salmen W]] | |||