6xmr: Difference between revisions

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-'''Unreleased structure'''
-The entry 6xmr is ON HOLD
+==X-ray crystallographic structure model of Lactococcus lactis prolidase mutant H38S==
+<StructureSection load='6xmr' size='340' side='right'caption='[[6xmr]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6xmr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XMR FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xmr OCA], [https://pdbe.org/6xmr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xmr RCSB], [https://www.ebi.ac.uk/pdbsum/6xmr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xmr ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A8WBX8_9LACT A8WBX8_9LACT]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Lactococcus lactis prolidase (Xaa-Pro dipeptidase; EC.3.4.13.9) is unique from other prolidases by showing allosteric behaviour, substrate inhibition, and metal-dependent substrate specificity. We have previously shown several critical residues for these characteristics using site-directed mutagenesis and amino acid sequence-based models. In this present study, the three-dimensional structure of recombinant L. lactis prolidase was determined by X-ray crystallography at 2.25A resolution in order to provide evidences of the proposed mechanism. Three molecules are located in the crystal asymmetric unit where molecule A forms a dimer with molecule B, while molecule C forms a dimer with molecule C' in the adjacent asymmetric unit. Of all the three molecules, molecule C is less defined and incomplete. While this fact compromises the overall quality of the refined model, the functional interpretation of the structure is not compromised since the biologically-functional homodimeric configuration of L. lactis prolidase is represented by well-defined molecules A and B. The refined model confirmed that there is a twelve-residue (residues 32-43) loop structure from one subunit over the active site of the other subunit, proving the existence of the putative loop structure in our previous study. This loop is three amino acids longer than the loops of prolidases of Pyrococcus furious (1pv9) and Pyrococcus horikoshii OT3 (1wy2). The crystal structure shows the loop structure can form two states ("open" and "closed" states) through interaction between the loop and active site proximity. It supports the proposed formation of allosteric site by the loop and Arg 293.
-Authors: Xu, S., Grochulski, P., Tanaka, T.
+Crystallographic structure of recombinant Lactococcus lactis prolidase to support proposed structure-function relationships.,Kgosisejo O, Chen JA, Grochulski P, Tanaka T Biochim Biophys Acta Proteins Proteom. 2017 May;1865(5):473-480. doi:, 10.1016/j.bbapap.2017.02.004. Epub 2017 Feb 4. PMID:28179139<ref>PMID:28179139</ref>
-Description: X-ray crystallographic structure model of Lactococcus lactis prolidase mutant H38S
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Grochulski, P]]
+<div class="pdbe-citations 6xmr" style="background-color:#fffaf0;"></div>
-[[Category: Xu, S]]
+== References ==
-[[Category: Tanaka, T]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Lactococcus lactis]]
+[[Category: Large Structures]]
+[[Category: Grochulski P]]
+[[Category: Tanaka T]]
+[[Category: Xu S]]