6wi4: Difference between revisions
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The | ==Caspases from Scleractinian Coral== | ||
<StructureSection load='6wi4' size='340' side='right'caption='[[6wi4]], [[Resolution|resolution]] 1.57Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6wi4]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Porites_astreoides Porites astreoides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WI4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.57Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wi4 OCA], [https://pdbe.org/6wi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wi4 RCSB], [https://www.ebi.ac.uk/pdbsum/6wi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wi4 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Coral reefs are experiencing precipitous declines around the globe with coral diseases and temperature-induced bleaching being primary drivers of these declines. Regulation of apoptotic cell death is an important component in the coral stress response. Although cnidaria are known to contain complex apoptotic signaling pathways, similar to those in vertebrates, the mechanisms leading to cell death are largely unexplored. We identified and characterized two caspases each from Orbicella faveolata, a disease-sensitive reef-building coral, and Porites astreoides, a disease-resistant reef-building coral. The caspases are predicted homologs of the human executioner caspases-3 and -7, but OfCasp3a (Orbicella faveolata caspase-3a) and PaCasp7a (Porites astreoides caspase-7a), which we show to be DxxDases, contain an amino-terminal caspase activation/recruitment domain (CARD) similar to human initiator/inflammatory caspases. OfCasp3b (Orbicella faveolata caspase-3b) and PaCasp3 (Porites astreoides caspase-3), which we show to be VxxDases, have short pro-domains, like human executioner caspases. Our biochemical analyses suggest a mechanism in coral which differs from that of humans, where the CARD-containing DxxDase is activated on death platforms but the protease does not directly activate the VxxDase. The first X-ray crystal structure of a coral caspase, of PaCasp7a determined at 1.57A resolution, reveals a conserved fold and an N-terminal peptide bound near the active site that may serve as a regulatory exosite. The binding pocket has been observed in initiator caspases of other species. These results suggest mechanisms for the evolution of substrate selection while maintaining common activation mechanisms of CARD-mediated dimerization. | |||
Caspases from Scleractinian Coral Show Unique Regulatory Features.,Shrestha S, Tung J, Grinshpon RD, Swartz P, Hamilton PT, Dimos B, Mydlarz L, Clark AC J Biol Chem. 2020 Aug 11. pii: RA120.014345. doi: 10.1074/jbc.RA120.014345. PMID:32788218<ref>PMID:32788218</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6wi4" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Caspase 3D structures|Caspase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Porites astreoides]] | |||
[[Category: Clark AC]] | |||
[[Category: Swartz PD]] | |||
Latest revision as of 17:25, 18 October 2023
Caspases from Scleractinian CoralCaspases from Scleractinian Coral
Structural highlights
Publication Abstract from PubMedCoral reefs are experiencing precipitous declines around the globe with coral diseases and temperature-induced bleaching being primary drivers of these declines. Regulation of apoptotic cell death is an important component in the coral stress response. Although cnidaria are known to contain complex apoptotic signaling pathways, similar to those in vertebrates, the mechanisms leading to cell death are largely unexplored. We identified and characterized two caspases each from Orbicella faveolata, a disease-sensitive reef-building coral, and Porites astreoides, a disease-resistant reef-building coral. The caspases are predicted homologs of the human executioner caspases-3 and -7, but OfCasp3a (Orbicella faveolata caspase-3a) and PaCasp7a (Porites astreoides caspase-7a), which we show to be DxxDases, contain an amino-terminal caspase activation/recruitment domain (CARD) similar to human initiator/inflammatory caspases. OfCasp3b (Orbicella faveolata caspase-3b) and PaCasp3 (Porites astreoides caspase-3), which we show to be VxxDases, have short pro-domains, like human executioner caspases. Our biochemical analyses suggest a mechanism in coral which differs from that of humans, where the CARD-containing DxxDase is activated on death platforms but the protease does not directly activate the VxxDase. The first X-ray crystal structure of a coral caspase, of PaCasp7a determined at 1.57A resolution, reveals a conserved fold and an N-terminal peptide bound near the active site that may serve as a regulatory exosite. The binding pocket has been observed in initiator caspases of other species. These results suggest mechanisms for the evolution of substrate selection while maintaining common activation mechanisms of CARD-mediated dimerization. Caspases from Scleractinian Coral Show Unique Regulatory Features.,Shrestha S, Tung J, Grinshpon RD, Swartz P, Hamilton PT, Dimos B, Mydlarz L, Clark AC J Biol Chem. 2020 Aug 11. pii: RA120.014345. doi: 10.1074/jbc.RA120.014345. PMID:32788218[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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