3b0k: Difference between revisions

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-{{STRUCTURE_3b0k|  PDB=3b0k  |  SCENE=  }}
-===Crystal structure of alpha-lactalbumin===
-{{ABSTRACT_PUBMED_23155056}}
-==Function==
+==Crystal structure of alpha-lactalbumin==
-[[http://www.uniprot.org/uniprot/LALBA_CAPHI LALBA_CAPHI]] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
+<StructureSection load='3b0k' size='340' side='right'caption='[[3b0k]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3b0k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B0K FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b0k OCA], [https://pdbe.org/3b0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b0k RCSB], [https://www.ebi.ac.uk/pdbsum/3b0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b0k ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LALBA_CAPHI LALBA_CAPHI] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Addition of an extra methionine at the N-terminus by recombinant expression of alpha-lactalbumin in Escherichia coli significantly destabilizes the protein, and this destabilization has hampered mutational analyses such as the mutational phi-value analysis of the protein. Deletion of residue 1 from the recombinant form recovers the stability in human and goat alpha-lactalbumin. Here, we thus determined the crystal structures of the residue 1-deletion variants of recombinant human and goat alpha-lactalbumin, and compared the structures with those of the authentic and recombinant forms. The results demonstrate the importance of the N-terminal backbone structure and hydrogen-bonding pattern for the stability of alpha-lactalbumin.
-==About this Structure==
+Structural insights into the stability perturbations induced by N-terminal variation in human and goat alpha-lactalbumin.,Makabe K, Nakamura T, Kuwajima K Protein Eng Des Sel. 2013 Feb;26(2):165-70. doi: 10.1093/protein/gzs093. Epub, 2012 Nov 14. PMID:23155056<ref>PMID:23155056</ref>
-[[3b0k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/African_dwarf_goat African dwarf goat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B0K OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3b0k" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[Lactalbumin|Lactalbumin]]
+*[[Alpha-lactalbumin 3D structures|Alpha-lactalbumin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:023155056</ref><references group="xtra"/><references/>
+__TOC__
-[[Category: African dwarf goat]]
+</StructureSection>
-[[Category: Makabe, K.]]
+[[Category: Capra hircus]]
-[[Category: Calcium binding protein]]
+[[Category: Large Structures]]
-[[Category: Glycoprotein]]
+[[Category: Makabe K]]
-[[Category: Metal binding protein]]