6vo9: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6vo9 is ON HOLD  until Paper Publication
+==Artificial Metalloproteins with Dinuclear Iron Centers==
+<StructureSection load='6vo9' size='340' side='right'caption='[[6vo9]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6vo9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VO9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VO9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=KM3:{N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+)'>KM3</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vo9 OCA], [https://pdbe.org/6vo9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vo9 RCSB], [https://www.ebi.ac.uk/pdbsum/6vo9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vo9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Dinuclear iron centers with a bridging hydroxido or oxido ligand form active sites within a variety of metalloproteins. A key feature of these sites is the ability of the protein to control the structures around the Fe centers, which leads to entatic states that are essential for function. To simulate this controlled environment, artificial proteins have been engineered using biotin-streptavidin (Sav) technology in which Fe complexes from adjacent subunits can assemble to form [Fe(III)-(mu-OH)-Fe(III)] cores. The assembly process is promoted by the site-specific localization of the Fe complexes within a subunit through the designed mutation of a tyrosinate side chain to coordinate the Fe centers. An important outcome is that the Sav host can regulate the Fe...Fe separation, which is known to be important for function in natural metalloproteins. Spectroscopic and structural studies from X-ray diffraction methods revealed uncommonly long Fe...Fe separations that change by less than 0.3 A upon the binding of additional bridging ligands. The structural constraints imposed by the protein host on the di-Fe cores are unique and create examples of active sites having entatic states within engineered artificial metalloproteins.
-Authors:
+Artificial Metalloproteins with Dinuclear Iron-Hydroxido Centers.,Miller KR, Biswas S, Jasniewski A, Follmer AH, Biswas A, Albert T, Sabuncu S, Bominaar EL, Hendrich MP, Moenne-Loccoz P, Borovik AS J Am Chem Soc. 2021 Feb 10;143(5):2384-2393. doi: 10.1021/jacs.0c12564. Epub 2021 , Feb 2. PMID:33528256<ref>PMID:33528256</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6vo9" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Avidin 3D structures|Avidin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Streptomyces avidinii]]
+[[Category: Albert T]]
+[[Category: Biswas S]]
+[[Category: Borovik AS]]
+[[Category: Follmer AH]]
+[[Category: Hendrich MP]]
+[[Category: Jasniewski AJ]]
+[[Category: Miller KR]]
+[[Category: Moenne-Loccoz P]]
+[[Category: Sabuncu S]]