6ud5: Difference between revisions

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-'''Unreleased structure'''
-The entry 6ud5 is ON HOLD  until Paper Publication
+==Crystal structure of human tryptophan 2,3-dioxygenase in complex with carbon monoxide and tryptophan==
+<StructureSection load='6ud5' size='340' side='right'caption='[[6ud5]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6ud5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UD5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UD5 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ud5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ud5 OCA], [https://pdbe.org/6ud5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ud5 RCSB], [https://www.ebi.ac.uk/pdbsum/6ud5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ud5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/T23O_HUMAN T23O_HUMAN] Incorporates oxygen into the indole moiety of tryptophan. Has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Human indoleamine 2,3-dioxygenase 1 (hIDO1) and human tryptophan dioxygenase (hTDO) are two important heme proteins that degrade the essential amino acid, l-tryptophan (Trp), along the kynurenine pathway. The two enzymes share a similar active site structure and an analogous catalytic mechanism, but they exhibit a variety of distinct functional properties. Here we used carbon monoxide (CO) as a structural probe to interrogate how the functionalities of the two enzymes are encoded in their structures. With X-ray crystallography, we detected an unexpected photochemical intermediate trapped in a crystal of the hIDO1-CO-Trp complex, where CO is photolyzed from the heme iron by X-rays at cryogenic temperatures (100 K). The CO photolysis triggers a large-scale migration of the substrate Trp, as well as the photolyzed CO, from the active site to a temporary binding site, Sa*. It is accompanied by a large conformational change to an active site loop, JK-Loop(C), despite the severely restricted protein motion under the frozen conditions, which highlights the remarkable conformational plasticity of the hIDO1 protein. Comparative studies of a crystal of the hTDO-CO-Trp complex show that CO and Trp remain bound in the active site under comparable X-ray illumination, indicating a much more rigid protein architecture. The data offer important new insights into the structure and function relationships of the heme-based dioxygenases and provide new guidelines for structure-based design of inhibitors targeting them.
-Authors: Pham, K.N., Lewis-Ballester, A., Yeh, S.R.
+Conformational Plasticity in Human Heme-Based Dioxygenases.,Pham KN, Lewis-Ballester A, Yeh SR J Am Chem Soc. 2020 Dec 29. doi: 10.1021/jacs.0c09970. PMID:33373218<ref>PMID:33373218</ref>
-Description: Crystal structure of human tryptophan 2,3-dioxygenase in complex with carbon monoxide and tryptophan
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Yeh, S.R]]
+<div class="pdbe-citations 6ud5" style="background-color:#fffaf0;"></div>
-[[Category: Pham, K.N]]
-[[Category: Lewis-Ballester, A]]
+==See Also==
+*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Lewis-Ballester A]]
+[[Category: Pham KN]]
+[[Category: Yeh SR]]