6q1c: Difference between revisions
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The | ==Apo YfeA extracted from the E. coli periplasm== | ||
<StructureSection load='6q1c' size='340' side='right'caption='[[6q1c]], [[Resolution|resolution]] 1.76Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6q1c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q1C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Q1C FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6q1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q1c OCA], [https://pdbe.org/6q1c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6q1c RCSB], [https://www.ebi.ac.uk/pdbsum/6q1c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6q1c ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/YFEA_YERPE YFEA_YERPE] Part of an ATP-driven transport system YfeABCD for chelated iron. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In the structural biology of bacterial substrate-binding proteins (SBPs), a growing number of comparisons between substrate-bound and substrate-free forms of metal atom-binding (cluster A-I) SBPs have revealed minimal structural differences between forms. These observations contrast with SBPs that bind substrates such as amino acids or nucleic acids and may undergo >60 degrees rigid-body rotations. Substrate transfer in these SBPs is described by a Venus flytrap model, although this model may not apply to all SBPs. In this report, structures are presented of substrate-free (apo) and reconstituted substrate-bound (holo) YfeA, a polyspecific cluster A-I SBP from Yersinia pestis. It is demonstrated that an apo cluster A-I SBP can be purified by fractionation when co-expressed with its cognate transporter, adding an alternative strategy to the mutagenesis or biochemical treatment used to generate other apo cluster A-I SBPs. The apo YfeA structure contains 111 disordered protein atoms in a mobile helix located in the flexible carboxy-terminal lobe. Metal binding triggers a 15-fold reduction in the solvent-accessible surface area of the metal-binding site and reordering of the 111 protein atoms in the mobile helix. The flexible lobe undergoes a 13.6 degrees rigid-body rotation that is driven by a spring-hammer metal-binding mechanism. This asymmetric rigid-body rotation may be unique to metal atom-binding SBPs (i.e. clusters A-I, A-II and D-IV). | |||
Structures of the substrate-binding protein YfeA in apo and zinc-reconstituted holo forms.,Radka CD, Labiuk SL, DeLucas LJ, Aller SG Acta Crystallogr D Struct Biol. 2019 Sep 1;75(Pt 9):831-840. doi: , 10.1107/S2059798319010866. Epub 2019 Aug 22. PMID:31478906<ref>PMID:31478906</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6q1c" style="background-color:#fffaf0;"></div> | ||
[[Category: Aller | == References == | ||
[[Category: Labiuk | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Yersinia pestis]] | |||
[[Category: Aller SG]] | |||
[[Category: DeLucas LJ]] | |||
[[Category: Labiuk SL]] | |||
[[Category: Radka CD]] | |||