6pbk: Difference between revisions

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-'''Unreleased structure'''
-The entry 6pbk is ON HOLD
+==Archaellum periplasmic stator protein complex FlaF and FlaG from Sulfolobus acidocaldarius==
+<StructureSection load='6pbk' size='340' side='right'caption='[[6pbk]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6pbk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PBK FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.805&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pbk OCA], [https://pdbe.org/6pbk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pbk RCSB], [https://www.ebi.ac.uk/pdbsum/6pbk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pbk ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q4J9K7_SULAC Q4J9K7_SULAC]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Motility structures are vital in all three domains of life. In Archaea, motility is mediated by the archaellum, a rotating type IV pilus-like structure that is a unique nanomachine for swimming motility in nature. Whereas periplasmic FlaF binds the surface layer (S-layer), the structure, assembly and roles of other periplasmic components remain enigmatic, limiting our knowledge of the archaellum's functional interactions. Here, we find that the periplasmic protein FlaG and the association with its paralogue FlaF are essential for archaellation and motility. Therefore, we determine the crystal structure of Sulfolobus acidocaldarius soluble FlaG (sFlaG), which reveals a beta-sandwich fold resembling the S-layer-interacting FlaF soluble domain (sFlaF). Furthermore, we solve the sFlaG2-sFlaF2 co-crystal structure, define its heterotetrameric complex in solution by small-angle X-ray scattering and find that mutations that disrupt the complex abolish motility. Interestingly, the sFlaF and sFlaG of Pyrococcus furiosus form a globular complex, whereas sFlaG alone forms a filament, indicating that FlaF can regulate FlaG filament assembly. Strikingly, Sulfolobus cells that lack the S-layer component bound by FlaF assemble archaella but cannot swim. These collective results support a model where a FlaG filament capped by a FlaG-FlaF complex anchors the archaellum to the S-layer to allow motility.
-Authors: Tsai, C.-L., Tainer, J.A.
+The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility.,Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV Nat Microbiol. 2020 Jan;5(1):216-225. doi: 10.1038/s41564-019-0622-3. Epub 2019, Dec 16. PMID:31844299<ref>PMID:31844299</ref>
-Description: Archaellum periplasmic stator protein complex FlaF and FlaG from Sulfolobus acidocaldarius
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Tsai, C.-L]]
+<div class="pdbe-citations 6pbk" style="background-color:#fffaf0;"></div>
-[[Category: Tainer, J.A]]
+==See Also==
+*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Sulfolobus acidocaldarius DSM 639]]
+[[Category: Tainer JA]]
+[[Category: Tsai C-L]]