6nt0: Difference between revisions

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-'''Unreleased structure'''
-The entry 6nt0 is ON HOLD
+==Catalase 3 from N.Crassa in ferrous state, X-ray reduced (1.315 MGy)==
+<StructureSection load='6nt0' size='340' side='right'caption='[[6nt0]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6nt0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa_OR74A Neurospora crassa OR74A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NT0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nt0 OCA], [https://pdbe.org/6nt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nt0 RCSB], [https://www.ebi.ac.uk/pdbsum/6nt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nt0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CAT3_NEUCR CAT3_NEUCR] Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Catalases are biotechnologically relevant enzymes because of their applications in food technology, bioremediation, and biomedicine. The dismutation of hydrogen peroxide occurs in two steps; in the first one, the enzyme forms an oxidized compound I (Cpd I) and in the second one, the enzyme is reduced to the ferric state. In this research work, we analyzed the reduction of Cpd I by X-ray radiation damage during diffraction experiments in crystals of CAT-3, a Large-Size Subunit Catalase (LSC) from Neurospora crassa. A Multi-Crystal Data collection Strategy was applied in order to obtain the Cpd I structure at a resolution of 2.2A; this intermediate was highly sensitive to X-ray and was easily reduced at very low deposited radiation dose, causing breakage of the Fe=O bond. The comparison of the structures showed reduced intermediates and also evidenced the differential sensitivity per monomer. The resting ferric state was reduced to the ferrous state, an intermediate without a previous report in LSC. The chemically obtained Cpd I and the X-ray reduced intermediates were identified by UV-visible microspectrometry coupled to data collection. The differential sensitivity and the formation of a ferrous state are discussed, emphasizing the importance of the correct interpretation in the oxidation state of the iron heme.
-Authors: Zarate-Romero, A., Rudino-Pinera, E., Stojanoff, V.
+X-ray driven reduction of Cpd I of Catalase-3 from N. crassa reveals differential sensitivity of active sites and formation of ferrous state.,Zarate-Romero A, Stojanoff V, Cohen AE, Hansberg W, Rudino-Pinera E Arch Biochem Biophys. 2019 Mar 30;666:107-115. doi: 10.1016/j.abb.2019.03.020. PMID:30940570<ref>PMID:30940570</ref>
-Description: Catalase 3 from N.Crassa in ferrous state, X-ray reduced (1.315 MGy)
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Stojanoff, V]]
+<div class="pdbe-citations 6nt0" style="background-color:#fffaf0;"></div>
-[[Category: Rudino-Pinera, E]]
-[[Category: Zarate-Romero, A]]
+==See Also==
+*[[Catalase 3D structures|Catalase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Neurospora crassa OR74A]]
+[[Category: Rudino-Pinera E]]
+[[Category: Stojanoff V]]
+[[Category: Zarate-Romero A]]