6e5x: Difference between revisions
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==Crystal structure of Ebola virus VP30 C-terminus/RBBP6 peptide complex== | ==Crystal structure of Ebola virus VP30 C-terminus/RBBP6 peptide complex== | ||
<StructureSection load='6e5x' size='340' side='right' caption='[[6e5x]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='6e5x' size='340' side='right'caption='[[6e5x]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6e5x]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6e5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Zaire_(1995) Ebola virus - Zaire (1995)] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E5X FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e5x OCA], [https://pdbe.org/6e5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e5x RCSB], [https://www.ebi.ac.uk/pdbsum/6e5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e5x ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/RBBP6_HUMAN RBBP6_HUMAN] E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 6e5x" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6e5x" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Amarasinghe GK]] | ||
[[Category: | [[Category: Leung DW]] | ||
[[Category: | [[Category: Liu D]] | ||
[[Category: | [[Category: Small GI]] | ||
Latest revision as of 09:19, 11 October 2023
Crystal structure of Ebola virus VP30 C-terminus/RBBP6 peptide complexCrystal structure of Ebola virus VP30 C-terminus/RBBP6 peptide complex
Structural highlights
FunctionRBBP6_HUMAN E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth. Publication Abstract from PubMedEbola virus (EBOV) infection often results in fatal illness in humans, yet little is known about how EBOV usurps host pathways during infection. To address this, we used affinity tag-purification mass spectrometry (AP-MS) to generate an EBOV-host protein-protein interaction (PPI) map. We uncovered 194 high-confidence EBOV-human PPIs, including one between the viral transcription regulator VP30 and the host ubiquitin ligase RBBP6. Domain mapping identified a 23 amino acid region within RBBP6 that binds to VP30. A crystal structure of the VP30-RBBP6 peptide complex revealed that RBBP6 mimics the viral nucleoprotein (NP) binding to the same interface of VP30. Knockdown of endogenous RBBP6 stimulated viral transcription and increased EBOV replication, whereas overexpression of either RBBP6 or the peptide strongly inhibited both. These results demonstrate the therapeutic potential of biologics that target this interface and identify additional PPIs that may be leveraged for novel therapeutic strategies. Protein Interaction Mapping Identifies RBBP6 as a Negative Regulator of Ebola Virus Replication.,Batra J, Hultquist JF, Liu D, Shtanko O, Von Dollen J, Satkamp L, Jang GM, Luthra P, Schwarz TM, Small GI, Arnett E, Anantpadma M, Reyes A, Leung DW, Kaake R, Haas P, Schmidt CB, Schlesinger LS, LaCount DJ, Davey RA, Amarasinghe GK, Basler CF, Krogan NJ Cell. 2018 Dec 13;175(7):1917-1930.e13. doi: 10.1016/j.cell.2018.08.044. PMID:30550789[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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