6cl5: Difference between revisions
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The | ==Structure of P. aeruginosa R1 pyocin fiber PALES_06171 comprising C-terminal residues 323-701== | ||
<StructureSection load='6cl5' size='340' side='right'caption='[[6cl5]], [[Resolution|resolution]] 2.32Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6cl5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_LESB58 Pseudomonas aeruginosa LESB58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CL5 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.324Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cl5 OCA], [https://pdbe.org/6cl5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cl5 RCSB], [https://www.ebi.ac.uk/pdbsum/6cl5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cl5 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9KW03_PSEAI Q9KW03_PSEAI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The R-type pyocins are high-molecular weight bacteriocins produced by some strains of Pseudomonas aeruginosa to specifically kill other strains of the same species. Structurally, the R-type pyocins are similar to "simple" contractile tails, such as those of phage P2 and Mu. The pyocin recognizes and binds to its target with the help of fibers that emanate from the baseplate structure at one end of the particle. Subsequently, the pyocin contracts its sheath and drives the rigid tube through the host cell envelope. This causes depolarization of the cytoplasmic membrane and cell death. The host cell surface-binding fiber is ~340 A-long and is attached to the baseplate with its N-terminal domain. Here, we report the crystal structures of C-terminal fragments of the R1 and R2 pyocin fibers that comprise the distal, receptor-binding part of the protein. Both proteins are ~240 A-long homotrimers in which slender rod-like domains are interspersed with more globular domains-two tandem knob domains in the N-terminal part of the fragment and a lectin-like domain at its C-terminus. The putative substrate binding sites are separated by about 100 A, suggesting that binding of the fiber to the cell surface causes the fiber to adopt a certain orientation relative to the baseplate and this then triggers sheath contraction. | |||
Structure and Analysis of R1 and R2 Pyocin Receptor-Binding Fibers.,Buth SA, Shneider MM, Scholl D, Leiman PG Viruses. 2018 Aug 14;10(8). pii: v10080427. doi: 10.3390/v10080427. PMID:30110933<ref>PMID:30110933</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6cl5" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas aeruginosa LESB58]] | |||
[[Category: Buth SA]] | |||
[[Category: Leiman PG]] | |||
[[Category: Shneider MM]] | |||
Latest revision as of 18:06, 4 October 2023
Structure of P. aeruginosa R1 pyocin fiber PALES_06171 comprising C-terminal residues 323-701Structure of P. aeruginosa R1 pyocin fiber PALES_06171 comprising C-terminal residues 323-701
Structural highlights
FunctionPublication Abstract from PubMedThe R-type pyocins are high-molecular weight bacteriocins produced by some strains of Pseudomonas aeruginosa to specifically kill other strains of the same species. Structurally, the R-type pyocins are similar to "simple" contractile tails, such as those of phage P2 and Mu. The pyocin recognizes and binds to its target with the help of fibers that emanate from the baseplate structure at one end of the particle. Subsequently, the pyocin contracts its sheath and drives the rigid tube through the host cell envelope. This causes depolarization of the cytoplasmic membrane and cell death. The host cell surface-binding fiber is ~340 A-long and is attached to the baseplate with its N-terminal domain. Here, we report the crystal structures of C-terminal fragments of the R1 and R2 pyocin fibers that comprise the distal, receptor-binding part of the protein. Both proteins are ~240 A-long homotrimers in which slender rod-like domains are interspersed with more globular domains-two tandem knob domains in the N-terminal part of the fragment and a lectin-like domain at its C-terminus. The putative substrate binding sites are separated by about 100 A, suggesting that binding of the fiber to the cell surface causes the fiber to adopt a certain orientation relative to the baseplate and this then triggers sheath contraction. Structure and Analysis of R1 and R2 Pyocin Receptor-Binding Fibers.,Buth SA, Shneider MM, Scholl D, Leiman PG Viruses. 2018 Aug 14;10(8). pii: v10080427. doi: 10.3390/v10080427. PMID:30110933[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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