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==Crystal structure of Staphylococcus aureus Ketol-acid Reductosimerrase with hydroxyoxamate inhibitor 3== | ==Crystal structure of Staphylococcus aureus Ketol-acid Reductosimerrase with hydroxyoxamate inhibitor 3== | ||
<StructureSection load='6c55' size='340' side='right' caption='[[6c55]], [[Resolution|resolution]] 2.09Å' scene=''> | <StructureSection load='6c55' size='340' side='right'caption='[[6c55]], [[Resolution|resolution]] 2.09Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6c55]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C55 OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6c55]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_RF122 Staphylococcus aureus RF122]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C55 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EKA:[cyclohexyl(hydroxy)amino](oxo)acetic+acid'>EKA</scene>, <scene name='pdbligand=EKD:(cyclohexylamino)(oxo)acetic+acid'>EKD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EKA:[cyclohexyl(hydroxy)amino](oxo)acetic+acid'>EKA</scene>, <scene name='pdbligand=EKD:(cyclohexylamino)(oxo)acetic+acid'>EKD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c55 OCA], [https://pdbe.org/6c55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c55 RCSB], [https://www.ebi.ac.uk/pdbsum/6c55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c55 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/ILVC_STAAB ILVC_STAAB] Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | ||
==See Also== | |||
*[[Ketol-acid reductoisomerase 3D structures|Ketol-acid reductoisomerase 3D structures]] | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Guddat | [[Category: Staphylococcus aureus RF122]] | ||
[[Category: Kandale | [[Category: Guddat LW]] | ||
[[Category: McGeary | [[Category: Kandale A]] | ||
[[Category: Patel | [[Category: McGeary RP]] | ||
[[Category: Schenk | [[Category: Patel KM]] | ||
[[Category: Schmbri | [[Category: Schenk G]] | ||
[[Category: You | [[Category: Schmbri M]] | ||
[[Category: Zheng | [[Category: You L]] | ||
[[Category: Zheng S]] | |||
Latest revision as of 17:56, 4 October 2023
Crystal structure of Staphylococcus aureus Ketol-acid Reductosimerrase with hydroxyoxamate inhibitor 3Crystal structure of Staphylococcus aureus Ketol-acid Reductosimerrase with hydroxyoxamate inhibitor 3
Structural highlights
FunctionILVC_STAAB Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. See Also |
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