6bs6: Difference between revisions

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-'''Unreleased structure'''
-The entry 6bs6 is ON HOLD  until Jan 26 2019
+==SusG with mixed linkage amylosaccharide==
+<StructureSection load='6bs6' size='340' side='right'caption='[[6bs6]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6bs6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BS6 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.17&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900009:alpha-maltotriose'>PRD_900009</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bs6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bs6 OCA], [https://pdbe.org/6bs6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bs6 RCSB], [https://www.ebi.ac.uk/pdbsum/6bs6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bs6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SUSG_BACTN SUSG_BACTN] Alpha-amylase that cleaves starch into oligosaccharides before internalization for degradation, the first step in starch degradation.<ref>PMID:10572122</ref> <ref>PMID:11717282</ref> <ref>PMID:20159465</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bacteria that reside in the mammalian intestinal tract efficiently hydrolyze dietary carbohydrates, including starch, that escape digestion in the small intestine. Starch is an abundant dietary carbohydrate comprised of alpha1,4 and alpha1,6 linked glucose, yet mammalian intestinal glucoamylases cannot effectively hydrolyze starch that has frequent alpha1,6 branching as these structures hinder recognition and processing by alpha1,4-specific amylases. Here we present the structure of the cell surface amylase SusG from Bacteroides thetaiotaomicron complexed with a mixed linkage amylosaccharide generated from transglycosylation during crystallization. Although SusG is specific for alpha1,4 glucosidic bonds, binding of this new oligosaccharide at the active site demonstrates that SusG can accommodate alpha1,6 branch points at subsite -3 to -2, and also at subsite+1 adjacent to the site of hydrolysis, explaining how this enzyme may be able to process a wide range of limit dextrins in the intestinal environment. These data suggest that B. thetaiotaomicron and related organisms may have a selective advantage for amylosaccharide scavenging in the gut.
-Authors: Koropatkin, N.M., Cockburn, D.W.
+Structural basis for the flexible recognition of alpha-glucan substrates by Bacteroides thetaiotaomicron SusG.,Arnal G, Cockburn DW, Brumer H, Koropatkin NM Protein Sci. 2018 Mar 30. doi: 10.1002/pro.3410. PMID:29603462<ref>PMID:29603462</ref>
-Description: SusG with mixed linkage amylosaccharide
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Koropatkin, N.M]]
+<div class="pdbe-citations 6bs6" style="background-color:#fffaf0;"></div>
-[[Category: Cockburn, D.W]]
+==See Also==
+*[[Amylase 3D structures|Amylase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Bacteroides thetaiotaomicron VPI-5482]]
+[[Category: Large Structures]]
+[[Category: Cockburn DW]]
+[[Category: Koropatkin NM]]