6bbn: Difference between revisions

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-'''Unreleased structure'''
-The entry 6bbn is ON HOLD
+==Crystal structure of a curved tubulin complex induced by the kinesin-13 Kif2A==
+<StructureSection load='6bbn' size='340' side='right'caption='[[6bbn]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6bbn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BBN FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.514&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbn OCA], [https://pdbe.org/6bbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bbn RCSB], [https://www.ebi.ac.uk/pdbsum/6bbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbn ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Kinesin-13 proteins are major microtubule (MT) regulatory factors that catalyze removal of tubulin subunits from MT ends. The class-specific "neck" and loop 2 regions of these motors are required for MT depolymerization, but their contributing roles are still unresolved because their interactions with MT ends have not been observed directly. Here we report the crystal structure of a catalytically active kinesin-13 monomer (Kif2A) in complex with two bent alphabeta-tubulin heterodimers in a head-to-tail array, providing a view of these interactions. The neck of Kif2A binds to one tubulin dimer and the motor core to the other, guiding insertion of the KVD motif of loop 2 in between them. AMPPNP-bound Kif2A can form stable complexes with tubulin in solution and trigger MT depolymerization. We also demonstrate the importance of the neck in modulating ATP turnover and catalytic depolymerization of MTs. These results provide mechanistic insights into the catalytic cycles of kinesin-13.
-Authors: Allingham, J.S., Trofimova, D.
+Ternary complex of Kif2A-bound tandem tubulin heterodimers represents a kinesin-13-mediated microtubule depolymerization reaction intermediate.,Trofimova D, Paydar M, Zara A, Talje L, Kwok BH, Allingham JS Nat Commun. 2018 Jul 6;9(1):2628. doi: 10.1038/s41467-018-05025-7. PMID:29980677<ref>PMID:29980677</ref>
-Description: Crystal structure of a curved tubulin complex induced by the kinesin-13 Kif2A
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Trofimova, D]]
+<div class="pdbe-citations 6bbn" style="background-color:#fffaf0;"></div>
-[[Category: Allingham, J.S]]
+==See Also==
+*[[Kinesin 3D Structures|Kinesin 3D Structures]]
+*[[Tubulin 3D Structures|Tubulin 3D Structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Bos taurus]]
+[[Category: Escherichia coli]]
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Allingham JS]]
+[[Category: Trofimova D]]